High-mobility group box 1 is overexpressed in cervical carcinoma and promotes cell invasion and migration in vitro

Autor: Shulan Zhang, Xiaoao Pang, Yao Zhang
Rok vydání: 2016
Předmět:
0301 basic medicine
Cancer Research
Uterine Cervical Neoplasms
Apoptosis
Metastasis
Immunoenzyme Techniques
HeLa
0302 clinical medicine
Cell Movement
Tumor Cells
Cultured
HMGB1 Protein
Cervical cancer
biology
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
Middle Aged
Cell cycle
Prognosis
Oncology
Lymphatic Metastasis
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Female
Signal Transduction
Adult
Epithelial-Mesenchymal Transition
Adolescent
Blotting
Western
chemical and pharmacologic phenomena
Adenocarcinoma
Real-Time Polymerase Chain Reaction
Cervical intraepithelial neoplasia
Young Adult
03 medical and health sciences
Biomarkers
Tumor
medicine
Carcinoma
Humans
Neoplasm Invasiveness
RNA
Messenger
Epithelial–mesenchymal transition
Aged
Cell Proliferation
Neoplasm Staging
Cancer
Uterine Cervical Dysplasia
medicine.disease
biology.organism_classification
030104 developmental biology
Cancer research
Zdroj: Oncology Reports. 37:831-840
ISSN: 1791-2431
1021-335X
Popis: The present study aimed to investigate the expression of high-mobility group box 1 protein (HMGB1) in cervical carcinoma and explore whether or not HMGB1 promotes cervical carcinoma cell invasion and migration in vitro and the related mechanism. HMGB1, nuclear factor-κB (NF-κB), E-cadherin and N-cadherin protein expression was analyzed in tissues from 48 cervical carcinomas, 51 cervical intraepithelial neoplasia (CIN) and tissues from 24 healthy controls using immunohistochemistry. HeLa cells were treated with different concentrations of HMGB1 (0, 10, 100, and 1,000 ng/ml) at different time-points (0, 24, 48 and 72 h), and changes in cell morphology and biological behaviors were observed. Changes in the expression levels of E-cadherin, N-cadherin, NF-κB and the inhibitor κB (IκB) in the treated cells were detected by western blot analysis and real-time PCR. HMGB1 expression exhibited a gradually increasing trend in the normal cervical tissues, CIN and cervical cancer, and there was statistical significance between the three groups (P
Databáze: OpenAIRE
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