Systematic error and comparison of four methods for assessing the viability ofSaccharomyces cerevisiae suspensions
| Autor: | C. Delorme, Jean-Michel Bavouzet, Hélène Lange, Patricia Taillandier |
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| Přispěvatelé: | Laboratoire de génie chimique [ancien site de Basso-Cambo] (LGC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA), Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Laboratoire de Génie Chimique (LGC), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT), Institut National Polytechnique de Toulouse - INPT (FRANCE) |
| Rok vydání: | 1993 |
| Předmět: |
0106 biological sciences
Systematic error Microscope Plate count Biology 01 natural sciences Applied Microbiology and Biotechnology Biochemistry law.invention Microbiology 03 medical and health sciences [CHIM.GENI]Chemical Sciences/Chemical engineering law 010608 biotechnology Microscopy Fluorescence microscope Génie chimique [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Viability assay Génie des procédés 0303 health sciences Methylene blue Chromatography 030306 microbiology Esterase activity Experimental error Staining |
| Zdroj: | Biotechnology Techniques Biotechnology Techniques, Springer Verlag, 1993, 7 (3), pp.223-228. ⟨10.1007/BF02566152⟩ Biotechnology Techniques, 1993, 7 (3), pp.223-228. ⟨10.1007/BF02566152⟩ |
| ISSN: | 1573-6784 0951-208X |
| DOI: | 10.1007/bf02566152 |
| Popis: | International audience; Four methods for the determination of cell viability were compared: the plate count technique, the flow cytometer, and two microscopic numerations- one after methylene blue staining and the other one with epifluorescence. The experimental error of these techniques was for the first time estimated: 8% for both numerations under microscope and 13% for the plate count technique. The staining mechanisms were explained by comparing the numerations under microscope and the flow cytometer analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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