Perioperacijske promjene subpopulacija limfocita u bolesnika operiranih zbog kolorektalnog karcinoma
| Autor: | Izabela Siemińska, Michał Pędziwiatr, Piotr Major, Jarosław Baran, Andrzej Budzyński, Piotr Małczak, Mateusz Rubinkiewicz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
030213 general clinical medicine 0209 industrial biotechnology medicine.medical_specialty Colorectal cancer Lymphocyte Kolorektalna kirurgija Lymphocyte subsets T-lymphocytes regulatory Th17 cells Lymphocyte count Colorectal surgery 02 engineering and technology regulacijski 03 medical and health sciences 020901 industrial engineering & automation 0302 clinical medicine Immune system White blood cell Subpopulacije limfocita T-limfociti medicine Humans Perioperative Period Original Scientific Papers T-lymphocytes Aged Aged 80 and over business.industry FOXP3 General Medicine Perioperative Middle Aged Flow Cytometry medicine.disease Surgery medicine.anatomical_structure Colonic Neoplasms Th17 stanice Broj limfocita regulatory business T-limfociti regulacijski CD8 |
| Zdroj: | Acta Clinica Croatica Acta clinica Croatica Volume 58. Issue 2. |
| ISSN: | 0353-9466 1333-9451 |
| Popis: | Surgical procedure has immense impact on the immune balance. However, little is known about perioperative changes in T regulatory and Th17 lymphocyte subpopulations in patients undergoing colorectal resection. Patients with resectable colon cancer were enrolled in the study. Blood samples were obtained on two occasions, i.e. before the procedure and two days after the surgery. We also recruited a control group of young, healthy individuals. Lymphocyte subpopulations were analyzed with the use of flow cytometry. Investigated subpopulations consisted of total lymphocyte count, CD4+, CD8+, T regulatory Foxp3+ (Tregs), Th17 lymphocytes and white blood cell (WBC) count. There were significant differences in immune cell levels before and after the surgery. Reduction was recorded in the CD4+, CD8+, Tregs and total lymphocyte counts (p=0.002, p=0.01, p=0.008 and p=0.001, respectively). Increase was observed in total WBC and Th17 cells, however, Th17 lymphocytes did not reach statistical significance (p=0.01 and p=0.5, respectively). In conclusion, surgical intervention caused changes in all lymphocyte subpopulations investigated in patients undergoing surgery for colorectal cancer. However, it seemed to be an effect of perioperative trauma. Further studies are needed to investigate the impact of surgical intervention on lymphocyte subpopulations. Kirurški zahvat ima golem učinak na ravnotežu imunog sustava. Međutim, malo se zna o perioperacijskim promjenama u subpopulacijama T regulatornih i Th17 limfocita u bolesnika podvrgnutih resekciji kolorektuma. U istraživanje su bili uključeni bolesnici s resektabilnim karcinomom kolona. Uzorci krvi prikupljeni su dva puta: prije zahvata i dva dana nakon operacije. Uključena je bila i kontrolna skupina sastavljena od mladih i zdravih osoba. Subpopulacije limfocita analizirane su protočnom citometrijom. Istraživane su sljedeće subpopulacije: ukupan broj limfocita, CD4+, CD8+, T regulatorni Foxp3+ (Tregs), Th17 i bijela krvna slika. Utvrđene su značajne razlike u razinama imuno stanica prije i nakon operacije. Snižene razine zabilježene su za CD4+, CD8+, Tregs i ukupni broj limfocita (p=0,002, p=0,01, p=0,008 odnosno p=0,001). Povišenje je zapaženo za bijelu krvnu sliku i Th17 stanice, međutim, Th17 limfociti nisu postigli statističku značajnost (p=0,01 odnosno p=0,5). Zaključujemo da je kirurški zahvat uzrokovao promjene u svim istraživanim subpopulacijama limfocita kod bolesnika podvrgnutih operaciji kolorektalnog karcinoma. Međutim, čini se da je to bio učinak perioperacijske traume. Potrebna su daljnja istraživanja kako bi se ispitao utjecaj kirurškog zahvata na subpopulacije limfocita. |
| Databáze: | OpenAIRE |
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