LEUKOCYTE MITOCHONDRIAL ALTERATIONS AFTER CARDIAC SURGERY INVOLVING CARDIOPULMONARY BYPASS: CLINICAL CORRELATIONS
| Autor: | Lung-Ching Chen, Kelvin K C Tsai, Chen-Fu Hsu, Mei-Chun Huang, Chi-Woon Kong, Tai-Ger Hsu, Cheng-Hsiung Huang |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Resuscitation Cell Survival medicine.medical_treatment Inflammation Nitric Oxide Critical Care and Intensive Care Medicine Membrane Potentials law.invention Immune system Superoxides law Intensive care Leukocytes Cardiopulmonary bypass medicine Humans Interleukin 6 Aged Cardiopulmonary Bypass biology business.industry Thoracic Surgery Mitochondria Cytokine Bypass surgery Immunology Emergency Medicine biology.protein Female medicine.symptom business Biomarkers |
| Zdroj: | Shock. 21:315-319 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/00024382-200404000-00005 |
| Popis: | Cardiac surgery with the use of cardiopulmonary bypass (CPB) is known to initiate systemic inflammatory responses that are associated with immune dysregulations, but the pathomechanisms underlying these changes remain elusive. Mitochondrial transmembrane potential (MTP) is an important determinant of leukocytic functions and viability, and may be altered as a part of the cellular responses to systemic inflammatory insults. Therefore, we examined MTP in three subsets of peripheral leukocytes in 18 patients receiving uncomplicated cardiac surgery involving CPB. The MTP of neutrophils and lymphocytes significantly increased, whereas that of monocytes significantly declined, after the surgery. The alterations in leukocytic MTP were transient, normalizing 3 days to 1 week after the surgery, and were accompanied by transient overproduction of intracellular oxidants, including nitric oxide and superoxide. Despite these perturbations, the viability status of leukocytes remained unaltered. Positive correlations were found between the changes of leukocyte MTP and various clinical parameters, implying that leukocyte mitochondrial alterations are parts of the systemic immune perturbations induced by the bypass surgery. |
| Databáze: | OpenAIRE |
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