Characterisation of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis

Autor: Narciso Campos, Irene Pateraki, Andréa Hemmerlin, Albert Cairó, Manuel Rodríguez-Concepción, Iván Ahumada, Victor Gonzalez, Thomas J. Bach, Albert Boronat
Přispěvatelé: Ministerio de Educación y Ciencia (España), Generalitat de Catalunya, Agencia Española de Cooperación Internacional para el Desarrollo, European Commission
Rok vydání: 2008
Předmět:
Zdroj: Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
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ISSN: 1445-4408
DOI: 10.1071/fp08012
Popis: Thiolases are ubiquitous enzymes involved in many essential biochemical processes. Biosynthetic thiolases, also known as acetoacetyl-CoA thiolases (AACT), catalyse a reversible Claisen-type condensation of two acetyl-CoA molecules to form acetoacetyl-CoA. Here, we report the characterisation of two genes from Arabidopsis thaliana L., ACT1 and ACT2, which encode two closely related AACT isoforms (AACT1 and AACT2, respectively). Transient expression of constructs encoding AACT1 and AACT2 fused to GFP revealed that the two proteins show a different subcellular localisation. While AACT1 is found in peroxisomes, AACT2 localises in the cytosol and the nucleus. The peroxisomal localisation of AACT1 depends on the presence of a C-terminal peroxisomal targeting sequence (PTS1) motif (Ser-Ala-Leu) not previously found in other organisms. ACT1 and ACT2 genes are also differentially expressed. Whereas ACT2 is expressed at relatively high level in all plant tissues, the expression of ACT1 is restricted to roots and inflorescences and its transcript is present at very low levels. The obtained results are in agreement with the involvement of AACT2 in catalysing the first step of the mevalonate pathway. The metabolic function of AACT1 is not clear at present, although its particular peroxisomal localisation might exclude a role in isoprenoid biosynthesis.
This work was supported by grants from the Spanish Ministerio de Educación y Ciencia (BIO2006–03704 to A.B., BFU2006–14655 to N.C. and BIO2005–00367 to M.R.C., all including FEDER funds), and the Generalitat de Catalunya (grant 2005SGR-00914). This work has been carried out within the Centre CONSOLIDER on Agrigenomics funded by the Spanish Ministry of Education and Science. I.A. was recipient of a pre-doctoral MUTIS fellowship from the Agencia Española de Cooperación International.
Databáze: OpenAIRE
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