Characterization of heterochromatin by sequential counterstain-enhanced fluorescence in three domestic bird species: Meleagris gallopavo, Columba livia domestica, and Anser anser L
| Autor: | M. Kalat, A. Bigelbach, W. Schleger, B. Mayr, M. Lambrou |
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| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty Turkeys Heterochromatin Zoology Anser anser chemistry.chemical_compound Goose biology.animal Geese Genetics medicine Animals Columbidae Molecular Biology Genetics (clinical) Cells Cultured Fluorescent Dyes Base Composition biology Cytogenetics Chromosome Karyotype Fibroblasts biology.organism_classification Chromosome Banding chemistry Karyotyping Chromomycin A3 Female Meleagris gallopavo Biotechnology |
| Zdroj: | The Journal of heredity. 81(6) |
| ISSN: | 0022-1503 |
| Popis: | The karyotypes of three avian species--Meleagris gallopavo, Anser anser L., and Columba livia domestica--were investigated by means of counterstain-enhanced fluorescence techniques (chromomycin A3/distamycin A/DAPI followed by DAPI/actinomycin D staining). A heterochromatin characterization of macro- and microchromosomes was performed. CMA3-positive (GC-rich) regions in the turkey included the telomeres of chromosomes 1, 3, 4, and Z. In the goose, the chromosome 2 was also CMA3-bright at the telomeres. The W chromosome possessed large amounts of CMA3-bright material on the short arm in both the turkey and the goose. Two types of centromeric heterochromatin were distinguished on acro- to telocentric chromosomes 6 to 14 in the pigeon. The microchromosomal heterochromation of the turkey and goose was GC-rich but had a high degree of variation. In the pigeon, several microchromosomes possessed predominantly AT-rich heterochromatin. |
| Databáze: | OpenAIRE |
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