Developmental pathway of somatic embryogenesis from leaf tissues of macaw palm (Acrocomia aculeata) revealed by histological events
| Autor: | Jonny Everson Scherwinski-Pereira, Inaê Mariê de Araújo Silva-Cardoso, Zanderluce Gomes Luis, Filipe Sathler Meira |
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| Přispěvatelé: | FILIPE SATHLER MEIRA, UNB, ZANDERLUCE GOMES LUIS, UNIVERSIDADE FEDERAL DO SUL E SUDESTE DO PARÁ (UNIFESSPA), INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO, UNB, JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Somatic embryogenesis Picloram Plant Science Arecaceae 010603 evolutionary biology 01 natural sciences Macaw chemistry.chemical_compound Botany Morphogenesis Somatic tissues Ecology Evolution Behavior and Systematics Vascular tissue Ecology Acrocomia aculeata biology fungi Woody plants biology.organism_classification Vascular bundle Ontogenesis chemistry Callus Somatic embryos 010606 plant biology & botany Explant culture |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | This paper, in an unprecedented way, describes the ontogenesis of calli and somatic embryos of macaw palm (Acrocomia aculeata) from young leaf tissues of adult plants, aiming to elucidate the events underlying the process and consequent optimization of the technique. Leaves were inoculated in a callus induction medium, composed of Y3 salts, 2.5 g L−1 activated charcoal and 450 μM Picloram. For multiplication of the calli, they were separated from the explants and inoculated in a medium with the same composition as the previous phase. Histological analyses were performed on samples of immature leaves with and without calli at 0, 3, 15, 30, 60, 90 and 120 days in an induction medium, and on samples of the different types of calli and somatic embryos obtained during multiplication and differentiation. For this, the samples were fixed in FAA, dehydrated in an alcohol series and infiltrated in resin. Anatomical sections obtained on a microtome were stained with Toluidine Blue for structural characterization. At 60 days, analyses revealed the formation of callogenic masses in the vicinity of smaller vascular bundles. This multiplication approach allowed for the proliferation of the primary cell mass that evolved into new lineages of calli, especially the embryogenic yellowish nodular lineage, which originated somatic embryos. Additional histochemical analyses showed starch accumulation only in the adjacencies of areas undergoing intense cell division. The morpho-anatomic characterization allows the rapid identification of potential calli for the formation of somatic embryos from leaf tissues in macaw palm and promotes future investigations of the participation of vascular tissues in the events that precede somatic embryogenesis. |
| Databáze: | OpenAIRE |
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