Enhanced Radiosensitization by Liposome-Encapsulated Pimonidazole for Anticancer Effects on Human Melanoma Cells
| Autor: | Nobuhiko Miwa, Hiroshi Tanaka, Shinya Kato, Masatsugu Kimura, Katsuhiro Kageyama |
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| Rok vydání: | 2012 |
| Předmět: |
Radiation-Sensitizing Agents
Radiosensitizer Materials science Cell Survival Biomedical Engineering Bioengineering Radiation Tolerance Cell Line chemistry.chemical_compound Nanocapsules Materials Testing medicine Humans Pimonidazole General Materials Science Melanoma Liposome Cell growth General Chemistry Condensed Matter Physics medicine.disease chemistry Nitroimidazoles Cell culture Dipalmitoylphosphatidylcholine Liposomes Cancer research Intracellular |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 12:4472-4477 |
| ISSN: | 1533-4899 1533-4880 |
| DOI: | 10.1166/jnn.2012.6180 |
| Popis: | The nitroimidazole-related hypoxic radiosensitizer, pimonidazole (Pmz) was encapsulated in liposome composed of dipalmitoylphosphatidylcholine, cholesterol and dipalmitoylphosphatidylglycerol (molar ratio = 1:1:0.2; diameter = 112.9 nm), and the radiosensitization was evaluated in human melanoma cells HMV-II. Cell proliferation was examined by WST-8 assay after X-ray irradiation in the presence of liposomal Pmz or free-Pmz under hypoxic conditions. On 7th day after X-ray irradiation of 5 Gy, cell proliferation decreased more markedly in the administration of liposomal Pmz than free-Pmz at equivalent Pmz doses. Chromatin fragmentation or nuclear condensation was observed in liposomal Pmz-treated HMV-II cells. Radiosensitization was enhanced dose-dependently along with Pmz amounts of 250-2000 microM contained in liposomal Pmz. Intracellular uptake was more abundant for liposomal Pmz for 60-240 min than for free-Pmz. Thus liposomal Pmz has a potential to overcome radiation resistance in hypoxia, owing to enhanced intracellular uptake by melanoma cells. |
| Databáze: | OpenAIRE |
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