IDENTIFICATION OF CANINE VISCERAL LEISHMANIASIS IN A PREVIOUSLY UNAFFECTED AREA BY CONVENTIONAL DIAGNOSTIC TECHNIQUES AND CELL-BLOCK FIXATION
| Autor: | Maria de Fátima Madeira, Tuanne Rotti Abrantes, Valmir Laurentino Silva, Fabiano Borges Figueiredo, Denise Amaro da Silva, Carolina dos Santos F. S. Perié, Rodrigo Caldas Menezes, Artur Augusto Velho Mendes Junior |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030106 microbiology 030231 tropical medicine Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Biology Sensitivity and Specificity Chromatography Affinity Serology 03 medical and health sciences Dogs 0302 clinical medicine Canine visceral leishmaniasis Seroepidemiologic Studies Diagnosis parasitic diseases medicine Animals Parasite hosting Dog Diseases Leishmania infantum Fluorescent Antibody Technique Indirect Fixation (histology) medicine.diagnostic_test General Medicine medicine.disease biology.organism_classification Cross-Sectional Studies Infectious Diseases Visceral leishmaniasis Immunoassay Leishmaniasis Visceral Female Original Article Histopathology Brazil Cell-block |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 (2016) Revista do Instituto de Medicina Tropical de São Paulo, Volume: 58, Article number: 3, Published: 23 FEB 2016 Revista do Instituto de Medicina Tropical de São Paulo Revista do Instituto de Medicina Tropical de São Paulo; Vol. 58 (2016); e3 Revista do Instituto de Medicina Tropical de São Paulo; v. 58 (2016); e3 Instituto de Medicina Tropical (IMT) instacron:IMT |
| ISSN: | 0036-4665 1678-9946 |
| DOI: | 10.1590/s1678-9946201658003 |
| Popis: | After the report of a second case of canine visceral leishmaniasis (CVL) in São Bento da Lagoa, Itaipuaçu, in the municipality of Maricá, Rio de Janeiro State, an epidemiological survey was carried out, through active search, totaling 145 dogs. Indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), and rapid chromatographic immunoassay based on dual-path platform (DPP(r)) were used to perform the serological examinations. The parasitological diagnosis of cutaneous fragments was performed by parasitological culture, histopathology, and immunohistochemistry. In the serological assessment, 21 dogs were seropositive by IFA, 17 by ELISA, and 11 by DPP(r), with sensitivity of 66.7%, 66.7% and 50%, and specificity of 87.2%, 90.2% and 94%, respectively for each technique. The immunohistochemistry of bone marrow using the cell-block technique presented the best results, with six positive dogs found, three of which tested negative by the other parasitological techniques. Leishmania sp. was isolated by parasitological culture in three dogs. The detection of autochthonous Leishmania infantum in Itaipuaçu, and the high prevalence of seropositive dogs confirm the circulation of this parasite in the study area and alert for the risk of expansion in the State of Rio de Janeiro. |
| Databáze: | OpenAIRE |
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