Molecular characterization of Cryptosporidium in pigs in central Vietnam
| Autor: | Vu Vy Huynh, Rintaro Sato, Yasuhiro Fukuda, Chika Tada, Yutaka Nakai, Sam Thi Nguyen, Duc Tan Nguyen |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Genotype Swine animal diseases Molecular Sequence Data Cryptosporidiosis Cryptosporidium Biology DNA Ribosomal Polymerase Chain Reaction 18S ribosomal RNA Microbiology law.invention Feces Medical microbiology law parasitic diseases Prevalence RNA Ribosomal 18S medicine Animals Heat-Shock Proteins Polymerase chain reaction Swine Diseases Molecular Epidemiology General Veterinary Molecular epidemiology Genetic Variation Genes rRNA Sequence Analysis DNA General Medicine DNA Protozoan Ribosomal RNA biology.organism_classification Infectious Diseases Vietnam Insect Science Parasitology RNA Protozoan |
| Zdroj: | Parasitology Research. 112:187-192 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-012-3124-2 |
| Popis: | Little information is available on the epidemiology of Cryptosporidium in pigs in central Vietnam. The aims of this study were to investigate the prevalence and to characterize the genotype distribution of Cryptosporidium isolates in pigs in this region. A total of 193 pig fecal samples were screened for the presence of Cryptosporidium oocysts using the modified Ziehl-Neelsen staining method, and 28 (overall prevalence 14.5 %) were identified as positive by microscopic observation. Positive samples were further analyzed by polymerase chain reaction amplification and sequencing. Genetic identification based on the 18S ribosomal RNA and 70 kDa heat shock protein genes revealed that pigs in Vietnam are infected with two species/genotypes (Cryptosporidium suis and Cryptosporidium pig genotype II). This study is the first molecular characterization of Cryptosporidium in pigs in Vietnam. The presence of these host-adapted species/genotypes suggests that pigs may not pose a significant public health risk in this area. More extensive studies are necessary to ascertain the zoonotic potential of Cryptosporidium in porcine hosts in Vietnam. |
| Databáze: | OpenAIRE |
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