Automated DNA Extraction Monitoring System Based on MTConnect Technology
| Autor: | Ae-Ja Park, Mun-Ho Ryu, Ah-Reum Park, Sang-Ho Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0209 industrial biotechnology
MTConnect computer.internet_protocol Computer science Reliability (computer networking) Request–response 02 engineering and technology POCT device lcsh:Technology lcsh:Chemistry 020901 industrial engineering & automation General Materials Science lcsh:QH301-705.5 Instrumentation DNA extraction Fluid Flow and Transfer Processes Flexibility (engineering) lcsh:T Adapter (computing) business.industry Process Chemistry and Technology General Engineering Process (computing) molecular genetic test 021001 nanoscience & nanotechnology real-time monitoring lcsh:QC1-999 Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Embedded system Scalability lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology business computer lcsh:Physics XML |
| Zdroj: | Applied Sciences Volume 11 Issue 2 Applied Sciences, Vol 11, Iss 684, p 684 (2021) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app11020684 |
| Popis: | MTConnect standard technology provides simplicity, flexibility, and scalability in integrating various equipment and operating systems and enabling accurate and consistent data collection from any MTConnect-compatible system. Using MTConnect technology, it is possible to immediately identify the cause of a problem and respond quickly when a problem occurs. Molecular genetic diagnostic point-of-care testing (POCT) devices have received attention in recent years because they enable rapid disease diagnosis. A molecular genetic diagnostic POCT device is under development by the authors. The system consists of a gene extraction process and a real-time PCR-based gene amplification process. In this study, we propose and demonstrate a system based on MTConnect technology to monitor an automated DNA extraction process. The proposed system consists of an automated DNA extraction system, an MTConnect adapter, an MTConnect agent, and a client application. The adapter and agent were developed on a Raspberry Pi single-board computer. The agent publishes the collected data in Extensible Markup Language (XML) format over a network. The performance and reliability of the system were evaluated by verifying the request response time between the implemented system&rsquo s agent and the client application. The results demonstrate the feasibility of monitoring the DNA extraction process over a network. |
| Databáze: | OpenAIRE |
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