Microarray expression profiling identifies genes with altered expression in HDL-deficient mice
| Autor: | Matthew J. Callow, Sandrine Dudoit, Terence P. Speed, Elaine L. Gong, Edward M. Rubin |
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| Rok vydání: | 2000 |
| Předmět: |
Genetically modified mouse
CD36 Antigens Male Letter Microarray Transgene Mice Transgenic Biology Mice Gene expression Genetics Animals Receptors Immunologic Apolipoproteins C Genetics (clinical) Oligonucleotide Array Sequence Analysis Receptors Lipoprotein Regulation of gene expression Mice Knockout Receptors Scavenger Expressed sequence tag Apolipoprotein C-III Apolipoprotein A-I Gene Expression Profiling Membrane Proteins Scavenger Receptors Class B Molecular biology Gene expression profiling Mice Inbred C57BL Gene Expression Regulation Liver DNA microarray Lipoproteins HDL Oxidoreductases |
| Zdroj: | ResearcherID |
| ISSN: | 1088-9051 |
| Popis: | Based on the assumption that severe alterations in the expression of genes known to be involved in high-density lipoprotein (HDL) metabolism may affect the expression of other genes, we screened an array of >5000 mouse expressed sequence tags for altered gene expression in the livers of two lines of mice with dramatic decreases in HDL plasma concentrations. Labeled cDNA from livers of apolipoprotein AI (apoAI)-knockout mice, scavenger receptor BI (SR-BI) transgenic mice, and control mice were cohybridized to microarrays. Two-samplet statistics were used to identify genes with altered expression levels in the knockout or transgenic mice compared with control mice. In the SR-BI group we found nine array elements representing at least five genes that were significantly altered on the basis of an adjusted P value P |
| Databáze: | OpenAIRE |
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