Fibroblast growth factor 2 alters the oxytocin receptor in a developmental model of anxiety-like behavior in male rat pups
| Autor: | Mariel B. Ríos, Bruce S. McEwen, Sraboni Chaudhury, Peter Blandino, Yoav Litvin, Pamela M. Maras, Miriam E. R. Baker, Huda Akil, Cortney A. Turner, Stanley J. Watson |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty medicine.drug_class Anxiety Fibroblast growth factor Oxytocin Amygdala Anxiolytic Article Epigenesis Genetic Rats Sprague-Dawley 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Endocrinology Neurochemical Internal medicine Gene expression medicine Animals Behavior Animal Endocrine and Autonomic Systems Maternal Deprivation Central Amygdaloid Nucleus Gene Expression Regulation Developmental Oxytocin receptor Rats Stria terminalis 030104 developmental biology medicine.anatomical_structure Animals Newborn Receptors Oxytocin Female Fibroblast Growth Factor 2 Septal Nuclei Growth and Development Psychology 030217 neurology & neurosurgery medicine.drug |
| Popis: | We aimed to determine the short-term effects of early-life stress in the form of maternal separation (MS) on anxiety-like behavior in male rat pups. In order to assess anxiety, we measured 40 kHz separation-induced ultrasonic vocalizations (USV) on postnatal day (PND) 11. We further aimed to evaluate the potential involvement of two neurochemical systems known to regulate social and anxiety-like behaviors throughout life: oxytocin (OT) and fibroblast growth factor 2 (FGF2). For these purposes, we tested the effects of neonatal administration (on PND1) of an acute dose of FGF2 on USV and its potential interaction with MS. In addition, we validated the anxiolytic effects of OT and measured oxytocin receptor (OTR) gene expression, binding and epigenetic regulation via histone acetylation. Our results show that MS potentiated USV while acute administration of OT and FGF2 attenuated them. Further, we found that both FGF2 and MS increased OTR gene expression and the association of acH3K14 with the OTR promoter in the bed nucleus of the stria terminalis (BNST). Comparable changes, though not as pronounced, were also found for the central amygdala (CeA). Our findings suggest that FGF2 may exert its anxiolytic effects in male MS rats by a compensatory increase in the acetylation of the OTR promoter to overcome reduced OT levels in the BNST. |
| Databáze: | OpenAIRE |
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