Mouse Dipeptidyl Peptidase 4 Is Not a Functional Receptor for Middle East Respiratory Syndrome Coronavirus Infection
| Autor: | Mark T. Heise, Boyd Yount, Trevor Scobey, Nicole R. Curnes, Ralph S. Baric, Sudhakar Agnihothram, Adam S. Cockrell, Kayla M. Peck |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Middle East respiratory syndrome coronavirus Protein Conformation Dipeptidyl Peptidase 4 viruses DNA Mutational Analysis Molecular Sequence Data Immunology Virus Attachment Biology medicine.disease_cause Microbiology Host Specificity Mice Protein structure Species Specificity Virology medicine Animals Humans Amino Acid Sequence Receptor Peptide sequence Gene Dipeptidyl peptidase-4 Coronavirus chemistry.chemical_classification virus diseases Amino acid Virus-Cell Interactions chemistry Insect Science Receptors Virus Coronavirus Infections |
| Zdroj: | Journal of Virology |
| DOI: | 10.17615/v26a-qn52 |
| Popis: | Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold, we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse-adapted MERS-CoV for rapid assessment of therapeutics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|