ZW10 Binding Factor (ZWINT), a Direct Target of Mir-204, Predicts Poor Survival and Promotes Proliferation in Breast Cancer
| Autor: | Guangrong Zhou, Mingyang Shen, Zhengyuan Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Breast Neoplasms
030204 cardiovascular system & hematology Biology 03 medical and health sciences 0302 clinical medicine Breast cancer Downregulation and upregulation Lab/In Vitro Research microRNA medicine Humans Genes Tumor Suppressor 3' Untranslated Regions Cell Proliferation Regulation of gene expression Three prime untranslated region Cell Cycle Intracellular Signaling Peptides and Proteins Cell cycle process Nuclear Proteins General Medicine Cell cycle ZWINT medicine.disease Prognosis Immunohistochemistry Survival Analysis Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030220 oncology & carcinogenesis Cancer research Female |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND ZW10 binding factor (ZWINT) has been reported to be upregulated in various human cancers and predict worse survival. However, the expression profile, clinical significance, and biological role of ZWINT remains unclear in breast cancer. MATERIAL AND METHODS In this study, we investigated messenger RNA (mRNA) and protein expression levels of ZWINT in breast cancer tissues, and the prognostic value of ZWINT protein expression was validated in a cohort of breast cancer patients using immunohistochemistry analysis. Then, different bioinformatic analyses were combined to explore the potential cancer-related hallmark underlying ZWINT in breast cancer, and a series of experiments in vitro were performed to reveal the oncogenic role of ZWINT in breast cancer. RESULTS Significant upregulation of ZWINT was observed in breast cancer tissues compared to normal and para-tumor tissues and upregulation of ZWINT predicts poor prognosis in breast cancer patients. Additionally, ZWINT could promote breast cancer proliferation via cell cycle regulation, especially by influencing the expression of some critical cell cycle regulators involved in G1 phase and G1/S transition. Finally, miR-204 was identified as a tumor suppressor microRNA which directly targets a specific site in 3'-UTR of ZWINT. CONCLUSIONS Overall, our results indicated that miR-204/ZWINT/cell cycle process might play an important role in breast cancer progression. |
| Databáze: | OpenAIRE |
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