Retrovirus-like particles released from the human breast cancer cell line T47-D display type B- and C-related endogenous retroviral sequences
| Autor: | H. Skladny, Christine Leib-Mösch, Ruediger Hehlmann, F. Krieg-Schneider, A. Reichert, Wolfgang Seifarth |
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| Rok vydání: | 1995 |
| Předmět: |
Sequence analysis
viruses Molecular Sequence Data Immunology Breast Neoplasms Betaretrovirus Polymerase Chain Reaction Microbiology Homology (biology) Cell Line Retrovirus Virology Tumor Cells Cultured Humans Genomic library Amino Acid Sequence RNA Messenger DNA Primers Southern blot Genetics Base Sequence biology RNA-Directed DNA Polymerase biology.organism_classification Molecular biology Retroviridae Insect Science RNA Viral Human genome Gammaretrovirus Research Article |
| Zdroj: | Journal of Virology. 69:6408-6416 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.69.10.6408-6416.1995 |
| Popis: | The human mammary carcinoma cell line T47-D releases retrovirus-like particles of type B morphology in a steroid-dependent manner (I. Keydar, T. Ohno, R. Nayak, R. Sweet, F. Simoni, F. Weiss, S. Karby, R. Mesa-Tejada, and S. Spiegelman, Proc. Natl. Acad. Sci. USA 81:4188-4192, 1984). Furthermore, reverse transcriptase (RT) activity is found to be associated with particle preparations. Using a set of degenerate primers derived from a conserved region of retroviral pol genes, we repeatedly amplified three different retroviral sequences (MLN, FRD, and FTD) from purified T47-D particles in several RT-PCR experiments. Screening of a human genomic library and Southern blot analysis revealed that these sequences are of endogenous origin. ERV-MLN represents a multicopy family of human endogenous retroviral elements (HERVs) with two closely related copies and up to 20 more distantly related members. In contrast, ERV-FRD and ERV-FTD comprise only one copy and five to seven related elements per haploid human genome. DNA sequence analysis of the proviral pol region of ERV-MLN revealed an uninterrupted stretch of 241 amino acids that shows 65% identity with the RT of the type B-related HERV designated HERV-K10. ERV-FRD and ERV-FTD are defective type C-related HERVs. The pol gene of ERV-FRD displays a nucleotide homology of 54% to the gibbon ape leukemia virus, and the pol gene of ERV-FTD is about 67% homologous to members of the RTVL-I family of HERVs. Our results thus indicate that the retroviral particles released by the breast cancer cell line T47-D are probably generated by complementation of several endogenous proviruses and can package retroviral transcripts of different origins. |
| Databáze: | OpenAIRE |
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