Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture

Autor: Susan G. Elner, H. Kaz Soong, Patricia C. T. Chang, W. C. Parkinson, Gregory L. Sulik, Victor M. Elner
Rok vydání: 2009
Předmět:
Zdroj: Acta Ophthalmologica. 70:115-122
ISSN: 1755-375X
DOI: 10.1111/j.1755-3768.1992.tb02102.x
Popis: Low-level, steady electric fields of 6-10 voltskm stimulated directional orientation and translo- cation of cultured human retinal pigment epithelial cells. The orientative movements (galvanotropism) consisted of somatic elongation of the cells into spindle shapes, fol- lowed by pivotal alignment orthogonal to the field. The anodal edges of the cells underwent retraction of their plasmalemmal extensions, while the cathode edges and the longitudinal ends developed lamellipodia and ruf- fled membranes. These tropic movements were followed by a translocational movement (galvanotaxis) of the cells towards the cathode. Staining of these migrating cells for actin showed the accumulation of stress fibers at the leading (cathodal) edge, as well as at the longitudinal ends of the elongated somata. These results suggest that endogenous, biologically-generated electric fields (eg., injury currents) may play a role in the guidance and mi- gration of retinal pigment epithelial cells after retinal in- Directional shape changes and movements during cell migration are controlled by several extrinsic factors, such as contact inhibition, chemotaxis, haptotaxis, and contact guidance (Trinkaus 1982). In this study, we investigate yet another possible factor that may play a role in controlling cell mi- gration, namely electric (E) fields. The influence of physiological E-fields on the modulation of direc
Databáze: OpenAIRE
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