Structure elucidation and antitumor activity of a new polysaccharide from Maerkang Tricholoma matsutake
| Autor: | Yiling Hou, Xiang Ding, Bo Song, Xiang-Hui Yan, Wanru Hou |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine biological activity Antineoplastic Agents Polysaccharide Applied Microbiology and Biotechnology Mice Random Allocation 03 medical and health sciences chemistry.chemical_compound Residue (chemistry) Maerkang Tricholoma matsutake In vivo Cell Line Tumor Gene expression Animals Humans Molecular Biology Ecology Evolution Behavior and Systematics chemistry.chemical_classification biology Chemistry structure elucidation Tricholoma Fungal Polysaccharides Biological activity Neoplasms Experimental Cell Biology biology.organism_classification In vitro RAW 264.7 Cells 030104 developmental biology Biochemistry polysaccharide Galactose Research Paper Developmental Biology |
| Zdroj: | International Journal of Biological Sciences |
| ISSN: | 1449-2288 |
| DOI: | 10.7150/ijbs.18953 |
| Popis: | A new heteropolysaccharide was isolated from the fruiting bodies of Tricholoma matsutake which had a molecular weight of 12078 Da. The results of structural features analysis showed that T. matsutake polysaccharide, here named TMP-B, was mainly composed of α - D - glucose and α - D - galactose which ratios were 7:2 and had a backbone of 1, 4 - linked α - D - glucose which branches were mainly composed of two 6 - linked α - D - galactose residue, and the α - D - galactose was 1, 6 - linked. Antitumor activity results showed that heteropolysaccharide TMP-B could inhibit the growth of S180 tumor in vivo and promote the apoptosis of L929 cells in vitro. Immunoregulatory activity results showed that TMP-B could promote the proliferation of macrophages by affecting G0/G1 phase, S phases and G2/M phases and promote cytokines release and gene expression. The result of this study introduced Maerkang T. matsutake as a possible valuable source which helped to exhibit unique antitumor and immunoregulatory properties. |
| Databáze: | OpenAIRE |
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