Human placental microvilli as a source of antigen for the preparation of a polyclonal antibody directed against the LDL receptor
| Autor: | Martine Moreau, C. Mazière, Jacques Polonovski, E. Alsat, Véronique Barbu, J.C. Mazière, Sopia Goldstein, Muriel Meier |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Antiserum
Microvilli medicine.drug_class Placenta Blotting Western General Medicine Biology Fibroblasts Monoclonal antibody Biochemistry Molecular biology medicine.anatomical_structure Antigen Receptors LDL Polyclonal antibodies LDL receptor Antibody Formation medicine biology.protein Humans lipids (amino acids peptides and proteins) Fibroblast Lipoprotein |
| Zdroj: | Biochimie. 71(3) |
| ISSN: | 0300-9084 |
| Popis: | Polyclonal antibodies were prepared by immunization of rabbits with partially purified LDL receptor obtained from human placental microvilli. The antiserum reacted with membranes from human placental microvilli and human fibroblasts, as assessed by immunobinding studies. It also reacted with purified LDL receptors of both origins. The antiserum markedly inhibited 125I-labeled LDL binding to cultured human fibroblasts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|