Toxicity studies of coumarin 6-encapsulated polystyrene nanospheres conjugated with peanut agglutinin and poly(N-vinylacetamide) as a colonoscopic imaging agent in rats
| Autor: | Richard McClure, Tokio Kitamura, John C. Gore, Shinji Sakuma, Kohta Mohri, Wellington Pham, Ken-ichiro Hiwatari, Etsuo Tobita, Moe Shimosato, Seiji Koike, Tetsuya Ikejima, Hironori Kumagai |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Peanut agglutinin Pathology medicine.medical_specialty Colon Drinking Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering CHO Cells Conjugated system Pharmacology Article Eating Peanut Agglutinin chemistry.chemical_compound Cricetulus Coumarins Oral administration Acetamides Animals Humans Medicine General Materials Science Fluorescent Dyes biology business.industry Body Weight Rectum Colonoscopy Coumarin Imaging agent Rats Thiazoles chemistry Toxicity biology.protein N-Vinylacetamide Polystyrenes Molecular Medicine Polyvinyls Polystyrene Caco-2 Cells Colorectal Neoplasms business Nanospheres |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 11:1227-1236 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2015.02.003 |
| Popis: | We are investigating an imaging agent that detects early-stage primary colorectal cancer on the mucosal surface in real time under colonoscopic observation. The imaging agent, which is named the nanobeacon, is fluorescent nanospheres conjugated with peanut agglutinin and poly(N-vinylacetamide). Its potential use as an imaging tool for colorectal cancer has been thoroughly validated in numerous studies. Here, toxicities of the nanobeacon were assessed in rats. The nanobeacon was prepared according to the synthetic manner which is being established as the Good Manufacturing Practice-guided production. The rat study was performed in accordance with Good Laboratory Practice regulations. No nanobeacon treatment-related toxicity was observed. The no observable adverse effect levels (NOAEL) of the nanobeacon in 7-day consecutive oral administration and single intrarectal administration were estimated to be more than 1000mg/kg/day and 50mg/kg/day, respectively. We concluded that the nanobeacon could be developed as a safe diagnostic agent for colonoscopy applications.Colon cancer remains a major cause of death. Early detection can result in early treatment and thus survival. In this article, the authors tested potential systemic toxicity of coumarin 6-encapsulated polystyrene nanospheres conjugated with peanut agglutinin (PNA) and poly(N-vinylacetamide) (PNVA), which had been shown to bind specifically to colonic cancer cells and thus very promising in colonoscopic detection of cancer cells. |
| Databáze: | OpenAIRE |
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