The Effect Of Different Workout Programs On The Expression Of The Genes Related To Oxidative Stress And Immune System
| Autor: | Kenan Aycan, Yusuf Ozkul, Neşe Akpinar Kocakulak, Serpi Taheri, Elif Funda Sener |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
business.industry General Medicine TFAM medicine.disease_cause Peripheral blood mononuclear cell Interval training Endocrinology Internal medicine IL12A Statistical significance Gene expression medicine exercise gene expression immune system Oxidative stress business Biology Oxidative stress Biyoloji UCP3 |
| Zdroj: | Volume: 3, Issue: 1 40-46 Natural and Applied Sciences Journal |
| ISSN: | 2645-9000 |
| Popis: | Background: exercise conditions can affect the expression of genes. Our study investigated the effects of acute and chronic exercise on the expression of genes related to oxidative stress (Tfam,UCP2,UCP3 ) and immune system (IL-1α,IL-1β,IL-2,IL-8,IL12A,IL12B,TLR2,TLR4 ). Methods: was formed with 24 people: 12 healthy females and 12 healthy males. Maximal oxygen use capacities of the participants were determined by the Bruce test protocol at the beginning and end of the 8-week training program. After calculating their maximal oxygen use capacity, each participant was given a acute running exercise on the tread mill at the speed and incline that the participant would reach to his/her maxVO2 until exhausted. The same people was built to continuous runs (%50-70) for 8 weeks in a week, and two days of medium-term interval training program (%90-95). Peripheral blood samples were taken before and after acute exercise with immediately after chronic exercises. RNA isolation was performed using TRIzol Reagent from peripheral blood mononuclear cells. Gene expression was determined by Biomark Real-Time PCR (RT-PCR). Comparisons were performed by using two independent sample t-test and Mann-Whitney U for quantitative data and calculated gene expression values. The statistical significance level was taken as p |
| Databáze: | OpenAIRE |
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