Molecular Epidemiology Survey of Toxinogenic Clostridium perfringens Strain Types by Multiplex PCR
| Autor: | Ewa Augustynowicz, Anna Gzyl, Janusz Slusarczyk |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Genotype Clostridium perfringens Bacterial Toxins medicine.disease_cause Polymerase Chain Reaction Microbiology law.invention Mice law Multiplex polymerase chain reaction medicine Animals Humans Clostridiaceae Typing Polymerase chain reaction DNA Primers Molecular Epidemiology General Immunology and Microbiology biology Molecular epidemiology Toxin General Medicine biology.organism_classification Bacterial Typing Techniques Phenotype Infectious Diseases Injections Intravenous Clostridium Infections |
| Zdroj: | Scandinavian Journal of Infectious Diseases. 32:637-641 |
| ISSN: | 1651-1980 0036-5548 |
| DOI: | 10.1080/003655400459559 |
| Popis: | Toxin genotypes of 95 C. perfringens strains collected within a 45-year period were analysed by a multiplex PCR. A set of primers designed for 4 different genes encoding the alpha, beta, epsilon, and iota toxins was used in a single reaction with a sensitivity of gene detection of 200 fg for DNA extracted from pure culture. Most of the strains (97%) conformed to the A biotype, and the remaining to the C or E biotypes. For biotype determination, seroneutralization of lethality in mice was performed by intravenous injection. Toxin phenotype and genotype profile were concordant in 94% of strains. Our results documented the presence of rare toxin genotypes of C. perfringens in a Polish geographical region and indicated the suitability of multiplex PCR as a method supplementing classical techniques and providing better insight into the prevalence of toxinogenic C. perfringens strains. |
| Databáze: | OpenAIRE |
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