Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells
| Autor: | Miyoko Matsushima, Etsuko Hirose, Takaaki Hasegawa, Kenji Baba, Fumie Abe, Tsutomu Kawabe, Kenzo Takagi, Yui Ota, Miyuki Ogawa, Yoshinori Hasegawa |
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| Rok vydání: | 2009 |
| Předmět: |
Allergy
Rodent Metalloporphyrins NF-E2-Related Factor 2 Immunology Protoporphyrins Pharmacology Antioxidants Cell Degranulation Mice chemistry.chemical_compound biology.animal Hypersensitivity medicine Animals Mast Cells Enzyme Inhibitors Rats Wistar Cells Cultured Carbon Monoxide Mice Inbred BALB C biology Degranulation Bilirubin hemic and immune systems medicine.disease Enzyme assay Rats Heme oxygenase Basophilic Disease Models Animal Leukemia Leukemia Basophilic Acute chemistry Heme Oxygenase (Decyclizing) biology.protein Quercetin Heme Oxygenase-1 |
| Zdroj: | Inflammation Research. 58:705-715 |
| ISSN: | 1420-908X 1023-3830 |
| Popis: | We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells.The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry.Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells.These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway. |
| Databáze: | OpenAIRE |
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