Spatial and Temporal Localization of Flavonoid Metabolites in Strawberry Fruit (Fragaria × ananassa)
| Autor: | Wilfried Schwab, Henryk Flachowsky, Thomas Hoffmann, Magda-Viola Hanke, Dirk Hölscher, Bernd Schneider, Thilo C. Fischer, Ulrich S. Schubert, Anna C. Crecelius |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Transgene Flavonoid Fragaria 01 natural sciences Anthocyanidin reductase 03 medical and health sciences chemistry.chemical_compound RNA interference Flavonol synthase NADH NADPH Oxidoreductases Plant Proteins Flavonoids chemistry.chemical_classification Molecular Structure biology 010401 analytical chemistry food and beverages Catechin General Chemistry 0104 chemical sciences Horticulture 030104 developmental biology Enzyme chemistry Biochemistry Fruit biology.protein Oxidoreductases General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:3559-3568 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b00584 |
| Popis: | Flavonoids are important metabolites in strawberries (Fragaria × ananassa) because they accomplish an extensive collection of physiological functions and are valuable for human health. However, their localization within the fruit tissue has not been extensively explored. Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to shed light on the spatial distribution of flavonoids during fruit development. One wild-type (WT) and two transgenic lines were compared, wherein the transgenic enzymes anthocyanidin reductase (ANRi) and flavonol synthase (FLSi), respectively, were down-regulated using an RNAi-based silencing approach. In most cases, fruit development led to a reduction of the investigated flavonoids in the fruit tissue; as a consequence, they were exclusively present in the skin of mature red fruits. In the case of (epi)catechin dimer, both the ANRi and the WT phenotypes revealed low levels in mature red fruits, whereas the ANRi line bore the lowest relative concentration, as analyzed by liquid chromatography-electrospray ionization multiple-step mass spectrometry (LC-ESI-MS |
| Databáze: | OpenAIRE |
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