Simian Virus 40 Large-T Bypasses the Translational Block Imposed by the Phosphorylation of eIF-2α
| Autor: | Prithi Rajan, Olga Savinova, Sathyamangalam Swaminathan, Rosemary Jagus, Bayar Thimmapaya |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gene Expression Regulation
Viral Antigens Polyomavirus Transforming viruses Eukaryotic Initiation Factor-2 Protein Serine-Threonine Kinases environment and public health Cell Line 03 medical and health sciences eIF-2 Kinase Virology Chlorocebus aethiops Protein biosynthesis Animals Phosphorylation Protein kinase A 030304 developmental biology Regulation of gene expression 0303 health sciences EIF-2 kinase biology 030306 microbiology Translation (biology) biochemical phenomena metabolism and nutrition Molecular biology Protein kinase R 3. Good health enzymes and coenzymes (carbohydrates) Protein Biosynthesis biology.protein Signal transduction Signal Transduction |
| Zdroj: | Virology. 219(1):321-323 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1996.0255 |
| Popis: | One of the cellular defense mechanisms against virus infection is mediated by activating the interferon-induced, double-stranded-RNA-activated protein kinase, PKR. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor, elF-2, leading to cessation of protein synthesis. Viruses have evolved diverse strategies to counteract this cellular antiviral response. A majority of these strategies target PKR to prevent its activation. Recently, we showed that simian virus 40 (SV40) large-T antigen reverses PKR-mediated translational inhibition at a step downstream of PKR activation (Rajan et al., J. Virol. 69, 785--795, 1995). In this paper, we present evidence showing that SV40 can restore efficient translation in cells despite the elevated levels of phosphorylated elF-2 alpha resulting from PKR activation. Thus, SV40 large-T-mediated translational rescue occurs at a step downstream of elF-2 alpha phosphorylation. |
| Databáze: | OpenAIRE |
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