Salivary NUS1 and RCN1 Levels as Biomarkers for Oral Squamous Cell Carcinoma Diagnosis
| Autor: | Kengo Hashimoto, Toru Nagao, Sei Ueda, Kazuo Shimozato, Ichiro Oh-Iwa, Dai Shimizu, Mitsuo Goto, Shogo Hasegawa, Shuji Nomoto, Satoru Miyabe |
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| Rok vydání: | 2020 |
| Předmět: |
Oncology
Cancer Research Candidate gene medicine.medical_specialty Saliva Receptors Cell Surface General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Internal medicine Gene expression Biomarkers Tumor medicine Screening method Humans Basal cell Pharmacology Squamous Cell Carcinoma of Head and Neck business.industry Calcium-Binding Proteins Cancer medicine.disease stomatognathic diseases Head and Neck Neoplasms 030220 oncology & carcinogenesis Mouth Neoplasms Reticulocalbin 1 DNA microarray business Biomarkers Research Article |
| Zdroj: | In Vivo |
| ISSN: | 1791-7549 0258-851X |
| Popis: | Background/aim Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection. Materials and methods Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues. Results qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV. Conclusion A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC. |
| Databáze: | OpenAIRE |
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