Targeted deletion of the tybe IIb Na+-dependent Pi-co-transporter, NaPi-IIb, results in early embryonic lethality
| Autor: | Kazuma Tomizuka, Takeyoshi Yamashita, Motoo Takahashi, Yuri Shibasaki, Kazunori Hanaoka, Nobuaki Etoh, Makoto Kakitani, Michiko Hayasaka |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Biophysics Embryonic Development Gene Expression In situ hybridization Biology Sodium-Phosphate Cotransporter Proteins Type IIb Biochemistry Mice Placenta Internal medicine Gene expression medicine Extracellular Animals Yolk sac Molecular Biology Embryogenesis Cell Biology Embryonic stem cell Mice Mutant Strains Cell biology Endocrinology medicine.anatomical_structure Embryo Loss Female Endoderm Gene Deletion |
| Zdroj: | Biochemical and Biophysical Research Communications. 381:482-486 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2009.02.067 |
| Popis: | NaPi-IIb encodes a Na(+)-dependent Pi co-transporter, which is expressed in various adult tissues and mediates transport of extracellular Pi ions coupling with Na(+) ion. To define the role of NaPi-IIbin vivo, NaPi-IIb gene deficient mice were generated utilizing targeted mutagenesis, yielding viable, heterozygous NaPi-IIb mice. In contrast, homozygous NaPi-IIb mice died in utero soon after implantation, indicating that NaPi-IIb was essential for early embryonic development. In situ hybridization revealed NaPi-IIb mRNA expression in the parietal endoderm, followed by the visceral endoderm, at a time point prior to establishment of a functioning chorio-allantoic placenta. At the time point of functional placenta development, the main site of NaPi-IIb production resided in the labyrinthine zone, where embryonic and maternal circulations were in closest contact. Expression patterns of NaPi-IIb suggest that NaPi-IIb plays an important role in Pi absorption from maternal circulation. |
| Databáze: | OpenAIRE |
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