Cloning and expression of ATP N-glycosidase from the freshwater sponge Ephydatia muelleri
| Autor: | Kerli Vallmann, Nele Aas-Valleriani, Tõnu Reintamm, Annika Lopp, Mailis Päri, Kaidi Kolk, Merike Kelve |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Subfamily Glycoside Hydrolases Gene Expression Biochemistry Pichia Pichia pastoris 03 medical and health sciences Glycogen phosphorylase Protein Domains Animals Cloning Molecular chemistry.chemical_classification Cloning Messenger RNA 030102 biochemistry & molecular biology biology Chemistry General Medicine biology.organism_classification Sperm Recombinant Proteins Porifera Sponge 030104 developmental biology Enzyme |
| Zdroj: | Biochimie. 158 |
| ISSN: | 1638-6183 |
| Popis: | Previously we had discovered unusual enzymatic activity in the marine sponge Axinella polypoides, ATP N-glycosidase (Reintamm et al., 2003). We show here that the Ephydatia muelleri mRNA encoding protein with PNP_UDP_1 (phosphorylase superfamily) signature is the secreted ATP N-glycosidase. The functionality of the protein was established by recombinant expression in Pichia pastoris. In addition to the enzymatic domain, the full-length protein contains the N-terminal cysteine-rich domain belonging to the subfamily SCP_HrTT-1 (cd05559) of the SCP (sperm coating protein) superfamily (cl00133). |
| Databáze: | OpenAIRE |
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