Increased expression of secreted frizzled-related protein-1 and microtubule-associated protein light chain 3 in keratoconus
| Autor: | Nasir Sadeghi, Omer Iqbal, Samir Vira, Robert S. Feder, Daneyal Syed, Charles S. Bouchard, Edward M. Campbell, George Fisher, Joel Sugar, Jawed Fareed, David Freeman |
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| Rok vydání: | 2013 |
| Předmět: |
Keratoconus
genetic structures Corneal Stroma Biology Pathogenesis Stroma Secreted frizzled-related protein 1 medicine Autophagy Humans Expressivity (genetics) Endothelium Corneal Epithelium Corneal Membrane Proteins Anatomy medicine.disease Molecular biology Immunohistochemistry eye diseases Epithelium Tissue Donors Staining Ophthalmology medicine.anatomical_structure embryonic structures Intercellular Signaling Peptides and Proteins sense organs biological phenomena cell phenomena and immunity Microtubule-Associated Proteins |
| Zdroj: | Cornea. 32(5) |
| ISSN: | 1536-4798 |
| Popis: | PURPOSE To study the expression of secreted frizzled-related protein-1 (SFRP-1) and microtubule-associated protein light chain 3 (LC3), an autophagy marker, in keratoconus. METHODS Under an institutional review board-approved protocol, de-identified and/or surgically discarded normal donor (n = 10) and keratoconus corneas (n = 10) were obtained. The corneal samples were fixed in formalin and embedded in paraffin. Immunohistochemical staining using SFRP-1 and LC3 antibodies was performed. RESULTS The majority of expression of SFRP-1 was seen in the epithelium; however, in 3 tissues that showed high expression, staining was also present in the stroma and endothelium. Like SFRP-1, the LC3 expression in keratoconus tissues occurred at 3 different levels: low, medium, and high. Collectively these data suggest that there are differences in the expression levels of SFRP-1 and LC3 in keratoconus tissue compared with the normal tissue. Low expressivity of SFRP-1 seemed to correspond to low expressivity of LC3, whereas medium to high expressivity of SFRP-1 corresponded to medium to high expressivity of LC3. CONCLUSIONS Increased expression of SFRP-1 and LC3 was observed in keratoconus corneas. Keratocyte autophagy seen with keratoconus may play a role in the pathogenesis of keratoconus. |
| Databáze: | OpenAIRE |
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