Modulation of HLA class I antigen and ICAM-2 on endothelial cells after in vitro infection with human cytomegalovirus
| Autor: | Shapour Shahgasempour, Helen M. Garnett, Salwa B. Woodroffe |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Human cytomegalovirus
viruses T-Lymphocytes Immunology Cytomegalovirus Umbilical cord Cell Line Umbilical Cord Antigens CD HLA Antigens medicine Cell Adhesion Immunology and Allergy Humans biology Histocompatibility Antigens Class I Antibodies Monoclonal Cell Biology Adhesion medicine.disease Flow Cytometry Molecular biology In vitro medicine.anatomical_structure biology.protein HLA class I antigen Endothelium Vascular Antibody Cell Adhesion Molecules Intracellular |
| Zdroj: | Immunology and cell biology. 76(3) |
| ISSN: | 0818-9641 |
| Popis: | The aim of the present work is to investigate the expression of HLA class I antigen and intercellular adhesion molecule-2 (ICAM-2) on the surface of human umbilical cord endothelial cells (HUVEC) after infection with human cytomegalovirus (HCMV). The expression of HLA class I antigen and ICAM-2 were determined (using antibodies against HLA class I antigen and ICAM-2) by attachment inhibition assay and flow cytometric analysis. Attachment inhibition assay demonstrated that HCMV increased the expression of HLA class I antigen. This was confirmed by flow cytometric analysis, which showed an increase in HLA class I antigen expression on HCMV-infected HUVEC. The results of the expression of ICAM-2 using attachment inhibition assay revealed that ICAM-2 is involved significantly in the increased adhesion of T lymphocytes to HCMV-infected HUVEC. However, flow cytometric analysis revealed that there were no changes in the expression of ICAM-2 on HCMV-infected HUVEC. One possible explanation for this is that HCMV induces the activation of ICAM-2 on the surface of HCMV-infected endothelial cells without affecting its expression. |
| Databáze: | OpenAIRE |
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