Cloning and comparative analysis of the bovine, porcine, and equine sex chromosome genesZFXandZFY
| Autor: | Arkadi Poloumienko |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Molecular Sequence Data
Sus scrofa Kruppel-Like Transcription Factors Biology Y chromosome Conserved sequence Exon Genetics Animals Humans Coding region Amino Acid Sequence Horses Cloning Molecular Molecular Biology Gene Conserved Sequence X chromosome DNA Primers Zinc finger Sex Chromosomes Base Sequence Reverse Transcriptase Polymerase Chain Reaction Chromosome Zinc Fingers Sequence Analysis DNA General Medicine Molecular biology DNA-Binding Proteins Gene Components Cattle Transcription Factors Biotechnology |
| Zdroj: | Genome. 47:74-83 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/g03-099 |
| Popis: | A growing body of evidence suggests the involvement of sex chromosome genes in mammalian development. We report the cloning and characterization of the complete coding regions of the bovine Y chromosome ZFY and X chromosome ZFX genes, and partial coding regions of porcine and equine ZFX and ZFY genes. Bovine ZFY and ZFX are highly similar to each other and to ZFX and ZFY from other species. While bovine and human ZFY proteins are both 801 amino acids long, bovine ZFX is 5 amino acids shorter than human ZFX. Like in humans, both bovine ZFY and ZFX contain 13 zinc finger motifs and belong to the Krueppel family of C2H2-type zinc finger proteins. The internal exon–intron organization of the bovine, porcine and equine ZFX and ZFY genes has been determined and compared. Within this region, the exon lengths and the positions of the splice sites are conserved, further suggesting a high evolutionary conservation of the ZFX and ZFY genes. Additionally, new alternatively spliced forms of human ZFX have been identified.Key words: sex chromosome genes, zinc finger proteins, ZFX, ZFY, alternative splicing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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