Effects of microcurrent therapy on excisional elastic cartilage defects in young rats
| Autor: | Gláucia Maria Tech dos Santos, Edson Pereira Tangerino Filho, Israel Costa Vasconcelos, Andrea Aparecida de Aro, Fernanda Aparecida Sampaio Mendonça, José Luis Fachi, Marcelo Augusto Marretto Esquisatto, Edson Rosa Pimentel |
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| Rok vydání: | 2016 |
| Předmět: |
Cartilage
Articular Male 0301 basic medicine Pathology medicine.medical_specialty Decorin Type II collagen Connective tissue Elastic cartilage Biology Glycosaminoglycan 03 medical and health sciences Hydroxyproline chemistry.chemical_compound Chondrocytes 0302 clinical medicine medicine Animals Ear External Cell Proliferation Wound Healing 030222 orthopedics Electromagnetic Radiation Cartilage Elastic Cartilage Cell Differentiation Cell Biology General Medicine Rats 030104 developmental biology medicine.anatomical_structure chemistry Collagen Wound healing Developmental Biology |
| Zdroj: | Tissue and Cell. 48:224-234 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2016.03.004 |
| Popis: | The effects of microcurrent application on the elastic cartilage defects in the outer ear of young animals were analyzed. Sixty male Wistar rats were divided into a control (CG) and a treated group (TG). An excisional lesion was created in the right outer ear of each animal. Daily treatment was started after 24h and consisted of the application of a low-intensity (20μA) continuous electrical current to the site of injury for 5min. The animals were euthanized after 7, 14 and 28 days of injury and the samples were submitted to analyses. In CG, areas of newly formed cartilage and intense basophilia were seen at 28 days, while in TG the same observations were made already at 14 days. The percentage of birefringent collagen fibers was higher in CG at 28 days. The number of connective tissue cells and granulocytes was significantly higher in TG. Ultrastructural analysis revealed the presence of chondrocytes in TG at 14 days, while these cells were observed in CG only at 28 days. Cuprolinic blue staining and the amount of glycosaminoglycans were significantly higher in TG at 14 days and 28 days. The amount of hydroxyproline was significantly higher in TG at all time points studied. The active isoform of MMP-2 was higher activity in TG at 14 days. Immunoblotting for type II collagen and decorin was positive in both groups and at all time points. The treatment stimulated the proliferation and differentiation of connective tissue cells, the deposition of glycosaminoglycans and collagen, and the structural reorganization of these elements during elastic cartilage repair. |
| Databáze: | OpenAIRE |
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