First determination of the secondary structure of purified factor VIII light chain
| Autor: | Nicolas Bihoreau, J M Yon, Michel Desmadril, A Lehegarat, M P Fontaine-Aupart |
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| Rok vydání: | 1992 |
| Předmět: |
Factor VIII
Stereochemistry Hydrochloride Circular Dichroism Cell Biology Chromatography Ion Exchange Immunoglobulin light chain Biochemistry Protein Structure Secondary Dissociation (chemistry) Sequence identity chemistry.chemical_compound chemistry Humans Molecule Immunoadsorption Spectroscopy Molecular Biology Protein secondary structure Chromatography High Pressure Liquid Edetic Acid Immunosorbent Techniques Research Article |
| Zdroj: | Biochemical Journal. 288:35-40 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2880035 |
| Popis: | The first analysis of the secondary structure of human factor VIII light chain was performed by c.d. spectroscopy. The purification process described in this paper allowed us to obtain the large amounts of purified factor VIII light chains required for c.d. experiments. Since this 80 kDa protein is non-covalently associated with a heavy chain to form the active molecule, isolated factor VIII light chains were obtained after immunoadsorption and dissociation of the immobilized active complexes by EDTA. Furthermore, factor VIII light chains were discriminated from the residual active complexes and the free heavy chains by a final ion-exchange-chromatography step. This f.p.l.c. analysis showed that factor VIII light chains were less electronegative than the active complexes. The results of conformational analysis by c.d. show that the protein possesses a high degree of regular secondary structure (58%) with approx. 22% of alpha-helix and 36% of beta-strand structures. The protein was completely unfolded by 3 M-guanidine hydrochloride. The results obtained from the analysis of c.d. spectra were compared with those predicted from three different statistical methods based on amino-acid sequence. The secondary structure information obtained from these methods was in good agreement with the c.d. results. These results were comparable with the secondary structure prediction of ceruloplasmin, a protein known to show sequence identity to factor VIII. |
| Databáze: | OpenAIRE |
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