Asymptomatic Plasmodium malariae infections in children from suburban areas of Yaoundé, Cameroon
| Autor: | Amit Kumar, Vineeta Singh, Ngono Ngane Annie Rosalie, Mouelle Sone Albert, Dongang Nana Rodrigue Roman, Koanga Mogtomo Martin Luther |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Adolescent 030231 tropical medicine Plasmodium malariae Plasmodium Asymptomatic Polymerase Chain Reaction Giemsa stain law.invention 03 medical and health sciences 0302 clinical medicine law parasitic diseases medicine RNA Ribosomal 18S Humans 030212 general & internal medicine Cameroon Child Asymptomatic Infections Polymerase chain reaction Ssu rrna gene Microscopy biology Base Sequence Ribosomal RNA DNA Protozoan biology.organism_classification medicine.disease Virology Malaria Infectious Diseases Child Preschool Parasitology Female medicine.symptom Sequence Alignment |
| Zdroj: | Parasitology international. 67(1) |
| ISSN: | 1873-0329 |
| Popis: | The gold standard for malaria diagnosis is the microscopic examination of Giemsa stained thick blood smears though microscopy mostly may not detect the presence of Plasmodium species infections in asymptomatic samples. In the reported study, we used two diagnostic methods viz. the conventional microscopic examination and polymerase chain reaction (PCR) assay to analyse the asymptomatic malaria samples. PCR assay amplifying 18S small-subunit ribosomal RNA (SSU rRNA) gene of Plasmodium in 122 samples confirmed 68% of isolates as asymptomatic P. falciparum infections; with 87.9% mono-infections. We observed that the P. malariae positive samples were not diagnosed in microscopic examination of the blood smears but the PCR based diagnostic method revealed the presence of 12% P. malariae infections in asymptomatic samples from Yaounde region of Cameroon where no official cases of P. malariae have been reported for over a decade. The sequence analysis further confirmed the presence of 12% P. malariae in malaria positive samples with three base pair deletions and five substitutions in the SSU rRNA gene. |
| Databáze: | OpenAIRE |
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