Optimization of pilocarpine-mediated seizure induction in immunodeficient Nod-Scid mice
| Autor: | Sandra Ahn, Kwang-Soo Kim, Sangmi Chung, Amanda Leung, Yeachan Kim, George Savvidis, Maria Jose Luna, Miles G. Cunningham, Danielle Iskandar |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Xenotransplantation medicine.medical_treatment Video Recording Status epilepticus Mice SCID Pharmacology Article Temporal lobe Epilepsy Status Epilepticus medicine Animals Survival rate business.industry Pilocarpine Electroencephalography Single injection medicine.disease Immunohistochemistry Electrodes Implanted Disease Models Animal Neurology Epilepsy Temporal Lobe Anesthesia Mossy Fibers Hippocampal Female Neurology (clinical) medicine.symptom business medicine.drug |
| Popis: | Temporal lobe epilepsy (TLE) has been modeled in mice using pilocarpine induction, with variable results depending on specific strains. To allow efficient xenotransplantation for the purpose of optimizing potential cell-based therapy of human TLE, we have determined the optimal dosing strategy to produce spontaneous recurring seizures in immunodeficient NodScid mice. Multiple 100 mg/kg injections of pilocarpine have been shown to be more effective than single 300–400 mg/kg injections for inducing spontaneous seizures in NodScid mice. Under our optimal conditions, 88.1+/−2.9% of the mice experienced status epilepticus (SE) with a survival rate of 61.8+/−5.9%. Surviving SE mice displayed spontaneous recurrent seizures at a frequency of 2.8+/−0.9 seizures/day for a duration of 41.1+/−3.5 sec. The widely used method of a single injection of pilocarpine was significantly less efficient in inducing seizures in NodScid mice. Therefore, we have determined that a multiple injection “ramping up” of 100 mg/kg of pilocarpine is optimal for inducing TLE-like spontaneous seizures in NodScid mice. Using this method, mice with SE efficiently developed SRS and expressed mossy fiber sprouting, a signature histopathological feature of TLE. |
| Databáze: | OpenAIRE |
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