Profiling of tryptophan-related plasma indoles in patients with carcinoid tumors by automated, on-line, solid-phase extraction and HPLC with fluorescence detection
| Autor: | Elisabeth G.E. de Vries, Ido P. Kema, Pax H.B. Willemse, Bert Ooms, Wim G. Meijer, Gert Meiborg |
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| Přispěvatelé: | Guided Treatment in Optimal Selected Cancer Patients (GUTS), Lifestyle Medicine (LM) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Indole test
Analyte URINARY-EXCRETION Chromatography 5-Hydroxyindoleacetic acid Chemistry Carcinoid tumors PLATELET SEROTONIN CONTENT Biochemistry (medical) Clinical Biochemistry Tryptophan ELECTROCHEMICAL DETECTION medicine.disease BIOGENIC-AMINES DIAGNOSIS High-performance liquid chromatography PERFORMANCE LIQUID-CHROMATOGRAPHY METABOLITES SERUM 5-HYDROXYINDOLEACETIC ACID medicine Serotonin Solid phase extraction ENRICHMENT medicine.drug |
| Zdroj: | Clinical Chemistry, 47(10), 1811-1820. AMER ASSOC CLINICAL CHEMISTRY |
| ISSN: | 0009-9147 |
| Popis: | Background: Profiling of the plasma indoles tryptophan, 5-hydroxytryptophan (5-HTP), serotonin, and 5-hydroxyindoleacetic acid (5-HIAA) is useful in the diagnosis and follow-up of patients with carcinoid tumors. We describe an automated method for the profiling of these indoles in protein-containing matrices as well as the plasma indole concentrations in healthy controls and patients with carcinoid tumors. Methods: Plasma, cerebrospinal fluid, and tissue homogenates were prepurified by automated on-line solid-phase extraction (SPE) in Hysphere Resin SH SPE cartridges containing strong hydrophobic polystyrene resin. Analytes were eluted from the SPE cartridge by column switching. Subsequent separation and detection were performed by reversed-phase HPLC combined with fluorometric detection in a total cycle time of 20 min. We obtained samples from 14 healthy controls and 17 patients with metastasized midgut carcinoid tumors for plasma indole analysis. In the patient group, urinary excretion of 5-HIAA and serotonin was compared with concentrations of plasma indoles. Results: Within- and between-series CVs for indoles in platelet-rich plasma were 0.6–6.2% and 3.7–12%, respectively. Results for platelet-rich plasma serotonin compared favorably with those obtained by single-component analysis. Plasma 5-HIAA, but not 5-HTP was detectable in 8 of 17 patients with carcinoid tumors. In the patient group, platelet-rich plasma total tryptophan correlated negatively with platelet-rich plasma serotonin (P = 0.021; r = −0.56), urinary 5-HIAA (P = 0.003; r = −0.68), and urinary serotonin (P Conclusions: The present chromatographic approach reduces analytical variation and time needed for analysis and gives more detailed information about metabolic deviations in indole metabolism than do manual, single-component analyses. |
| Databáze: | OpenAIRE |
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