Chemokine CXCL 1 may serve as a potential molecular target for hepatocellular carcinoma
| Autor: | Hua Nian, Xiaodong Guo, Jie Chen, Xue-Qun He, Lei Wang, Ma‐wei Jiang, Quan-Gang Zhu, Xiao‐feng Zhai, Xue‐ming Zhang, Ke‐qi Han, Hui Han |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Chemokine Carcinoma Hepatocellular Chemokine CXCL1 Chemokine CXCL2 Interleukin-1beta Mice 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Gene expression medicine Animals Humans Radiology Nuclear Medicine and imaging Original Research Cancer Biology Oligonucleotide Array Sequence Analysis Tumor microenvironment Gene knockdown biology PCR array Gene Expression Profiling Liver Neoplasms hepatocellular carcinoma medicine.disease digestive system diseases Gene Expression Regulation Neoplastic CXCL1 CXCL2 030104 developmental biology CXCL3 Oncology siRNA 030220 oncology & carcinogenesis Hepatocellular carcinoma biology.protein Cancer research gene expression profile Chemokines Chemokines CXC Neoplasm Transplantation |
| Zdroj: | Cancer Medicine |
| ISSN: | 2045-7634 |
| DOI: | 10.1002/cam4.843 |
| Popis: | The purpose of this study was to screen for changes in chemokine and chemokine‐related genes that are expressed in hepatocellular carcinoma (HCC) as potential markers of HCC progression. Total RNA was extracted from tumor and peritumor tissues from mice with HCC and analyzed using a PCR microarray comprising 98 genes. Changes in gene expression of threefold or more were screened and subsequently confirmed by immunohistochemical analyses and western blotting. Furthermore, whether chemokine knockdown by RNA interference (RNAi) could significantly suppress tumor growth in vivo was also evaluated. Finally, total serum samples were collected from HCC patients with HBV/cirrhosis (n = 16) or liver cirrhosis (n = 16) and from healthy controls (n = 16). The serum mRNA and protein expression levels of CXCL1 in primary liver cancer patients were detected by qRT‐PCR and western blot analysis, respectively. Several genes were up‐regulated in tumor tissues during the progression period, including CXCL1, CXCL2, CXCL3, and IL‐1β, while CXCR1 expression was down‐regulated. CBRH‐7919 cells carrying CXCL1 siRNA resulted in decreased tumor growth in nude mice. The differences in serum CXCL1 mRNA and protein levels among the HCC, hepatic sclerosis (HS), and control groups were significant (P |
| Databáze: | OpenAIRE |
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