Laser Biomodulation on L 929 Cell Culture
Autor: | Aline Helena Araujo Machado, Renato Amaro Zângaro, Rodrigo Franco de Oliveira, Cristina Pacheco-Soares, Deise Aparecida de Almeida Pires-Oliveira |
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Rok vydání: | 2010 |
Předmět: |
Time Factors
Cell growth Lasers Endoplasmic reticulum Biomedical Engineering Tetrazolium Salts Fibroblasts Biology Mitochondrion Endoplasmic Reticulum DiOC6 Molecular biology Mitochondria Staining Mice chemistry.chemical_compound Microscopy Fluorescence chemistry Cell culture Fluorescence microscope Animals Radiology Nuclear Medicine and imaging MTT assay Cell Division Cells Cultured |
Zdroj: | Photomedicine and Laser Surgery. 28:167-171 |
ISSN: | 1557-8550 1549-5418 |
Popis: | The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm(2) and 50 mJ/cm(2)) on L929 fibroblast cells.Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important in vitro photobiomodulation on cell cultures and tissues.Irradiation was performed for three days at 24-h intervals. After each interval, the cells were stained with MitoTracker Orange and DioC6 dyes to assess the photobiomodulatory effects of irradiation on mitochondrial activity and changes in the endoplasmic reticulum. The MTT assay [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide] was used to evaluate cell proliferation.The fluorescence microscopy assessment of mitochondria and endoplasmic reticulum in cells irradiated with 6 J/cm(2) and 50 mJ/cm(2) demonstrated intense mitochondrial activity, which was confirmed by DioC6 staining. Reticular activity was observed stemming from increased protein synthesis. Photobiomodulation with 50 mJ/cm(2) was slightly higher than with 6 J/cm(2), as demonstrated by fluorescence microscopy results. Photobiomodulation was also time-dependent, with better results 72-h after irradiation. |
Databáze: | OpenAIRE |
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