Heterozygous mutations in GTP-cyclohydrolase-1 reduce BH4 biosynthesis but not pain sensitivity
| Autor: | Vibe Hellmund, Gösta Nachman, Sidsel Salling Thorborg, Erik Dupont, Cathrine Jespersgaard, Troels S. Jensen, Ole J. Bjerrum, Arafat Nasser, Jens Lykkesfeldt, Lisbeth Birk Møller, Anette Torvin Møller |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine GTPCH DOPA-responsive dystonia Cohort Studies chemistry.chemical_compound 0302 clinical medicine GTP Cyclohydrolase Cells Cultured Aged 80 and over Tetrahydrobiopterin INDUCTION Age Factors Middle Aged GTP cyclohydrolase 1 Neurology Dystonic Disorders Cytokines Female Gch1 gene GCH1 medicine.drug Adult Pain Threshold HYPERPHENYLALANINEMIA medicine.medical_specialty Genotype GTP cyclohydrolase I Pain Biology BIOPTERIN Neoptera Young Adult 03 medical and health sciences Sex Factors Biosynthesis Internal medicine medicine Animals Humans NITRIC-OXIDE SYNTHASE POLYMORPHISMS Aged Haplotype GTP-CYCLOHYDROLASE-I Fibroblasts Biopterin GENE 030104 developmental biology Anesthesiology and Pain Medicine Endocrinology chemistry Gtp cyclohydrolase PROTECTIVE HAPLOTYPE Mutation biology.protein Neurology (clinical) Capsaicin 030217 neurology & neurosurgery |
| Zdroj: | Nasser, A, Møller, A T, Hellmund, V, Thorborg, S S, Jespersgaard, C, Bjerrum, O J, Dupont, E, Nachman, G, Lykkesfeldt, J, Jensen, T S & Møller, L B 2018, ' Heterozygous mutations in GTP-cyclohydrolase-1 reduce BH4 biosynthesis but not pain sensitivity ', Pain, vol. 159, no. 6, pp. 1012-1024 . https://doi.org/10.1097/j.pain.0000000000001175 |
| Popis: | Human studies have demonstrated a correlation between noncoding polymorphisms of "the pain protective" haplotype in the GCH1 gene that encodes for GTP cyclohydrolase I (GTPCH1)-which leads to reduced tetrahydrobiopterin (BH4) production in cell systems-and a diminished perception of experimental and clinical pain. Here, we investigate whether heterozygous mutations in the GCH1 gene which lead to a profound BH4 reduction in patients with dopa-responsive dystonia (DRD) have any effect on pain sensitivity. The study includes an investigation of GCH1-associated biomarkers and pain sensitivity in a cohort of 22 patients with DRD and 36 controls. The patients with DRD had, when compared with controls, significantly reduced levels of BH4, neopterin, biopterin, and GTPCH1 in their urine, blood, or cytokine-stimulated fibroblasts, but their pain response with respect to non-painful stimulation, (acute) stimulus-evoked pain, or pain response after capsaicin-induced sensitization was not significantly different. A family-specific cohort of 11 patients with DRD and 11 controls were included in this study. The patients with DRD were heterozygous for the pain protective haplotype in cis with the GCH1 disease-causing mutation, c.899T>C. No effect on pain perception was observed for this combined haplotype. In conclusion, a reduced concentration of BH4 is not sufficient to alter ongoing pain sensitivity or evoked pain responses. |
| Databáze: | OpenAIRE |
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