Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization
| Autor: | U. Fröhlich, Manfred Gratzl, K. Sterzik, Georgia Lahr, Artur Mayerhofer, R. Zienecker |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lung Neoplasms
Granulosa cell Gene Expression Polymerase Chain Reaction Biochemistry Cell membrane Structural Biology Tumor Cells Cultured Carcinoma Small Cell NCAM Cell adhesion molecule Single-Strand Specific DNA and RNA Endonucleases Cell biology Neural cell adhesion molecule Folliculogenesis Luteinization medicine.anatomical_structure Adhesion Female Human Gene isoform endocrine system medicine.medical_specialty DNA Complementary Cell Adhesion Molecules Neuronal Molecular Sequence Data Biophysics Biology Corpus Luteum Luteal Cells Internal medicine Genetics medicine Humans RNA Messenger Molecular Biology Messenger RNA Granulosa Cells Base Sequence Cell Membrane Alternative splicing RNA Probes Cell Biology Microscopy Electron Endocrinology nervous system |
| Zdroj: | FEBS Letters. 346:207-212 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(94)00473-0 |
| Popis: | Freshly aspirated human granulosa cells from pre-ovulatory follicles and granulosa cells luteinized in culture possess the neural cell adhesion molecule (NCAM) of approximate molecular mass of 140,000 and NCAM mRNA as confirmed by S1-nuclease protection assays and RT-PCR. Moreover, in the process of luteinization the NCAM isoform pattern is modified. Isoforms containing an insert of 10 amino acids (termed VASE) in the extracellular domain of NCAM were supplemented by alternatively spliced isoforms without this insert. NCAM immunoreactivity, at light and electron microscope levels, was associated with the cell membrane of most granulosa cells which formed clusters. During time in culture an increasing subpopulation of granulosa cells, devoid of NCAM immunoreactivity, spread out and formed monolayers. This differential expression and the alternative splicing of NCAM during luteinization of granulosa cells raise the possibility that NCAM could be involved in folliculogenesis and the formation of the corpus luteum in the human. |
| Databáze: | OpenAIRE |
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