The Pseudomonas syringae type III effector HopF2 suppresses Arabidopsis stomatal immunity
| Autor: | Stephane Angers, David S. Guttman, Darrell Desveaux, Michael Wilton, Gitta Coaker, Jun Liu, Yulu C. Liu, Adam Mott, DongHyuk Lee, Brenden A. Hurley |
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| Přispěvatelé: | Vinatzer, Boris Alexander |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Proteomics General Science & Technology Plant Pathogens Arabidopsis lcsh:Medicine Plant Immunity Pseudomonas syringae chemical and pharmacologic phenomena Genetically Modified Plant Science 01 natural sciences 03 medical and health sciences Immune system Bacterial Proteins Immunity Botany Type III Secretion Systems Arabidopsis thaliana lcsh:Science 030304 developmental biology chemistry.chemical_classification ADP Ribose Transferases 0303 health sciences Reactive oxygen species Multidisciplinary biology Effector Plant Bacterial Pathogens lcsh:R Biology and Life Sciences Plant Disease Resistance biochemical phenomena metabolism and nutrition Plant Pathology Plants biology.organism_classification Plants Genetically Modified Cell biology chemistry Plant Stomata Host-Pathogen Interactions lcsh:Q 010606 plant biology & botany Research Article |
| Zdroj: | PloS one, vol 9, iss 12 PLoS ONE PLoS ONE, Vol 9, Iss 12, p e114921 (2014) |
| Popis: | Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases required for regulating stomatal aperture, suggesting HopF2 may manipulate stomatal immunity. Here we report HopF2 can inhibit stomatal immunity independent of its ADP-ribosyltransferase activity. Transgenic expression of HopF2 in Arabidopsis inhibits stomatal closing in response to P. syringae and increases the virulence of surface inoculated P. syringae. Further, transgenic expression of HopF2 inhibits flg22 induced reactive oxygen species production. Intriguingly, ADP-ribosyltransferase activity is dispensable for inhibiting stomatal immunity and flg22 induced reactive oxygen species. Together, this implies HopF2 may be a bifunctional T3SE with ADP-ribosyltransferase activity required for inhibiting apoplastic immunity and an independent function required to inhibit stomatal immunity. |
| Databáze: | OpenAIRE |
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