Isolation of bovine foamy virus in Japan
| Autor: | Yuma Hachiya, Keisuke Oguma, Kumiko Kimura, Mamiko Ono, Tetsuya Horikita, Hiroshi Sentsui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Virus Cultivation 040301 veterinary sciences viruses Cattle Diseases Genes env Virus 0403 veterinary science 03 medical and health sciences Retrovirus Antigen Japan Virology Animals Spumavirus Cells Cultured Phylogeny Syncytium Sheep General Veterinary biology Full Paper Goats 04 agricultural and veterinary sciences bovine foamy virus spumavirus Ouchterlony double immunodiffusion biology.organism_classification retrovirus 030104 developmental biology cattle syncytium |
| Zdroj: | SC10201903200004 NARO成果DBr-pub The Journal of Veterinary Medical Science |
| ISSN: | 0916-7250 |
| DOI: | 10.1292/jvms.18-0121 |
| Popis: | Bovine foamy virus (BFV) is endemic in many countries, but has not been reported in Japan. A syncytium-forming virus was isolated from peripheral blood leukocytes of clinically healthy cattle on a farm in Kanagawa prefecture during a periodic epidemiological survey of viral diseases. The isolate was propagated in primary fetal bovine muscle cells and subsequently passaged in Madin-Darby bovine kidney cells. Since the isolate appeared to be distinct from the viruses with syncytium-forming ability previously isolated in Japan, we attempted to identify it using genomic analyses and electron microscopy. A phylogenetic analysis revealed that the isolate belongs to the bovine foamy virus cluster and is highly similar to a BFV strain isolated in China. A sero-epidemiological survey was performed using agar gel immunodiffusion test with the isolated virus as the antigen, and five of the 57 cattle tested were found to be seropositive. |
| Databáze: | OpenAIRE |
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