Inhibitory Effects of Roseoside and Icariside E4 Isolated from a Natural Product Mixture (No-ap) on the Expression of Angiotensin II Receptor 1 and Oxidative Stress in Angiotensin II-Stimulated H9C2 Cells

Autor: Tae Yang Kim, Hanna Kang, Se-Chan Kim, Jung-Yun Lee, Jai Youl Ro, Eun Young Hong, Gwan Ui Hong, Young Ho Kim, Myung-Hee Chung, Young-In Kwon, Jae Yeo Park
Rok vydání: 2019
Předmět:
Angiotensin receptor
Pharmaceutical Science
roseoside
Stimulation
angiotensin II
Pharmacology
01 natural sciences
Analytical Chemistry
icariside E4
chemistry.chemical_compound
Glucosides
Drug Discovery
Glycosides
Chemokine CCL2
reactive oxygen species
chemistry.chemical_classification
0303 health sciences
Oxidase test
Superoxide
Chemistry (miscellaneous)
Molecular Medicine
Oxidation-Reduction
Nicotinamide adenine dinucleotide phosphate
hypertension
Lignans
Receptor
Angiotensin
Type 1
Article
Transforming Growth Factor beta1
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
Animals
Humans
RNA
Messenger
Physical and Theoretical Chemistry
030304 developmental biology
Biological Products
Reactive oxygen species
Angiotensin II receptor type 1
Tumor Necrosis Factor-alpha
Organic Chemistry
cardiomyocytes (H9C2 cells)
Hydrogen Peroxide
Angiotensin II
Rats
0104 chemical sciences
Oxidative Stress
010404 medicinal & biomolecular chemistry
Gene Expression Regulation
chemistry
No-ap (natural product mixture)
Norisoprenoids
Zdroj: Molecules, Vol 24, Iss 3, p 414 (2019)
Molecules
Volume 24
Issue 3
ISSN: 1420-3049
DOI: 10.3390/molecules24030414
Popis: Hypertension is a major risk factor for the development of cardiovascular diseases. This study aimed to elucidate whether the natural product mixture No-ap (NA) containing Pine densiflora, Annona muricate, and Monordica charantia, or its single components have inhibitory effects on hypertension-related molecules in Angiotensin II (Ang II)-stimulated H9C2 cells. Individual functional components were isolated and purified from NA using various columns and solvents, and then their structures were analyzed using ESI&ndash
MS, 1H-NMR, and 13H-NMR spectra. H9C2 cells were stimulated with 300 nM Ang II for 7 h. NA, telmisartan, ginsenoside, roseoside (Roseo), icariside E4 (IE4), or a combination of two components (Roseo and IE4) were administered to the cells 1 h before Ang II stimulation. The expression and activity of hypertension-related molecules or oxidative molecules were determined using RT-PCR, western blot, and ELISA. Ang II stimulation increased the expression of Ang II receptor 1 (AT1), tumor necrosis factor-&alpha
(TNF-&alpha
), monocyte chemoattractant protein-1 (MCP-1), tumor growth factor-&beta
(TGF-&beta
) mRNA, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and the levels of hydrogen peroxide (H2O2) and superoxide anion (&bull
O2&minus
) and reduced anti-oxidant enzyme activity. NA significantly improved the expression or activities of all hypertension-related molecules altered in Ang II-stimulated cells. Roseo or IE4 pretreatment either decreased or increased the expression or activities of all hypertension-related molecules similar to NA, but to a lesser extent. The pretreatment with a combination of Roseo and IE4 (1:1) either decreased or increased the expression of all hypertension-related molecules, compared to each single component, revealing a synergistic action of the two compounds. Thus, the combination of single components could exert promising anti-hypertensive effects similar to NA, which should be examined in future animal and clinical studies.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje