Biological and structural analyses of bovine heparin fractions of intermediate and high molecular weight
| Autor: | Daiana L. Drehmer, Thales R. Cipriani, Marcello Iacomini, Alexsandro Vinícius Nogueira, Guilherme L. Sassaki |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Polymers and Plastics medicine.drug_class Ultrafiltration Low molecular weight heparin 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine In vivo Materials Chemistry medicine Animals Chromatography biology Molecular mass Chemistry Heparin Organic Chemistry Anticoagulants Heparin Low-Molecular-Weight Protamine Bioavailability Molecular Weight 030104 developmental biology Biochemistry biology.protein Cattle Partial Thromboplastin Time Ex vivo medicine.drug |
| Zdroj: | Carbohydrate polymers. 157 |
| ISSN: | 1879-1344 |
| Popis: | Low molecular weight heparin, which is generally obtained by chemical and enzymatic depolymerization of unfractionated heparin, has high bioavailability and can be subcutaneously injected. The aim of the present investigation was to fractionate bovine heparin using a physical method (ultrafiltration through a 10kDa cut-off membrane), avoiding structural modifications that can be caused by chemical or enzymatic treatments. Two fractions with different molecular weights were obtained: the first had an intermediate molecular weight (B-IMWH; Mn=9587Da) and the other had a high molecular weight (B-HMWH; 22,396Da). B-IMWH and B-HMWH have anticoagulant activity of 103 and 154IU/mg respectively, which could be inhibited by protamine. Both fractions inhibited α-thrombin and factor Xa in vitro and showed antithrombotic effect in vivo. Moreover, ex vivo aPTT assay demonstrated that B-IMWH is absorbed by subcutaneous route. The results showed that ultrafiltration can be used to obtain two bovine heparin fractions, which differ on their molecular weights, structural components, anticoagulant potency, and administration routes. |
| Databáze: | OpenAIRE |
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