Practical fabrication of microfluidic platforms for live-cell microscopy
| Autor: | S. Jeffrey Dixon, Jaques S. Milner, Noelle M. Ochotny, David W. Holdsworth, Daniel Lorusso, Stephen M. Sims, Hristo N. Nikolov |
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| Rok vydání: | 2016 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Fabrication Materials science Cell Survival Microfluidics Biomedical Engineering Nanotechnology 02 engineering and technology 01 natural sciences chemistry.chemical_compound Mice Lab-On-A-Chip Devices Microscopy Animals Dimethylpolysiloxanes Molecular Biology Polydimethylsiloxane 010401 analytical chemistry technology industry and agriculture PDMS stamp 3T3 Cells Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences Nylons Membrane chemistry Hydrodynamics 0210 nano-technology Microwave |
| Zdroj: | Biomedical microdevices. 18(5) |
| ISSN: | 1572-8781 |
| Popis: | We describe a simple fabrication technique - targeted towards non-specialists - that allows for the production of leak-proof polydimethylsiloxane (PDMS) microfluidic devices that are compatible with live-cell microscopy. Thin PDMS base membranes were spin-coated onto a glass-bottom cell culture dish and then partially cured via microwave irradiation. PDMS chips were generated using a replica molding technique, and then sealed to the PDMS base membrane by microwave irradiation. Once a mold was generated, devices could be rapidly fabricated within hours. Fibronectin pre-treatment of the PDMS improved cell attachment. Coupling the device to programmable pumps allowed application of precise fluid flow rates through the channels. The transparency and minimal thickness of the device enabled compatibility with inverted light microscopy techniques (e.g. phase-contrast, fluorescence imaging, etc.). The key benefits of this technique are the use of standard laboratory equipment during fabrication and ease of implementation, helping to extend applications in live-cell microfluidics for scientists outside the engineering and core microdevice communities. |
| Databáze: | OpenAIRE |
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