Expression and Clinical Significance of miR-146a and Tumor Necrosis Factor Receptor-Associated Factor 6 (TRAF6) in Myasthenia Gravis Patient Serum
Autor: | Xiaohuang Liang, Yue Fu, Huiling Zhou, Mei Yan, Hui Rao |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Adult
Male medicine.medical_specialty Article Subject Gastroenterology General Biochemistry Genetics and Molecular Biology Internal medicine Myasthenia Gravis Respiratory muscle medicine Humans Clinical significance RNA Messenger Conserved Sequence TNF Receptor-Associated Factor 6 Messenger RNA General Immunology and Microbiology Genome Human business.industry Respiration Curve analysis Muscle weakness General Medicine medicine.disease Myasthenia gravis MicroRNAs Multivariate Analysis Medicine Female Tumor necrosis factor alpha medicine.symptom business Tumor necrosis factor receptor Research Article |
Zdroj: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
ISSN: | 2314-6133 |
DOI: | 10.1155/2021/5573469 |
Popis: | Objective. To investigate the expression and clinical significance of miR-146a and tumor necrosis factor receptor-associated factor 6 (TRAF6) in myasthenia gravis (MG) patient serum. Methods. The serum of 52 patients with MG and 60 healthy individuals was collected in our hospital. The expression of miR-146a and TRAF6 in serum was measured by real-time PCR (RT-PCR). Comparison among serum miR-146a and TRAF6 mRNA group clinical characteristics and assorted expressions was done with the correlation among the two groups evaluated. Logistics regression was used to analyze the effect of miR-146a and TRAF6 mRNA on MG development with the ROC curve applied for an investigation into the diagnostic role of miR-146a and TRAF6 mRNA expression in MG development. Results. miR-146a and TRAF6 mRNA were significantly increased in the patients with MG compared with the healthy controls. Significant differences were identified in respiratory muscle endurance, muscle weakness level, vital capacity, and maximal voluntary ventilation between the two groups. Additionally, correlation analysis has discovered a positive correlation between miR-146a and TRAF mRNA expression in patients with MG. miR-146a and TRAF6 mRNA are independent MG occurrence factors exhibited by multivariate analysis while areas under ROC curve (AUCs) of miR-146a and TRAF6 mRNA in MG diagnosis were established by ROC curve analysis with results being 0.782 and 0.703, correspondingly. Conclusion. miR-146a and TRAF6 mRNA are highly expressed in MG patients and can affect MG occurrence. miR-146a is a suitable candidate marker for diagnosing MG. |
Databáze: | OpenAIRE |
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