Simultaneous determination of four tanshinones insalvia miltiorrhiza by pressurized liquid extraction and capillary electrochromatography
| Autor: | Peng Li, Feng-Qing Yang, Shao-Ping Li, Yitao Wang |
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| Rok vydání: | 2007 |
| Předmět: |
Capillary electrochromatography
Chromatography Molecular Structure Plant Extracts Chemistry Calibration curve Solid Phase Extraction Extraction (chemistry) Quinones Analytical chemistry Reproducibility of Results Salvia miltiorrhiza Filtration and Separation Repeatability Phenanthrenes Analytical Chemistry Electrochromatography Capillary Electrochromatography Abietanes Sample preparation Furans Quantitative analysis (chemistry) Drugs Chinese Herbal |
| Zdroj: | Journal of Separation Science. 30:900-905 |
| ISSN: | 1615-9314 1615-9306 |
| Popis: | A pressurized liquid extraction (PLE) and CEC were developed for the simultaneous determination of four tanshinones (dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA) in Salvia miltiorrhiza. High extraction efficiency (98.5%) was achieved under the optimum PLE conditions. A good separation was obtained by using a Hypersil C18 capillary (3 microm, 100 microm/25 cm) with a mixture of 30 mM Tris-HCl (pH 8.5)-ACN (1:3, v/v) as BGE solution running at 20 kV and 20 degrees C within 12 min. All the calibration curves showed good linearity (r20.9958) within test ranges. The developed method showed good repeatability for the quantification of four investigated components in S. miltiorrhiza with intra- and interday variations of less than 4.4 and 6.8%, respectively. The validated method was successfully applied to quantify four tanshinones in S. miltiorrhiza, which is helpful to control the quality of S. miltiorrhiza. |
| Databáze: | OpenAIRE |
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