Separation of Dihydro-Isocoumarins and Dihydro-Stilbenoids from Hydrangea macrophylla ssp. serrata by Use of Counter-Current Chromatography

Autor: Johannes Wellmann, Beate Hartmann, Esther-Corinna Schwarze, Silke Hillebrand, Stephan I. Brueckner, Jakob Ley, Gerold Jerz, Peter Winterhalter
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Ion-mobility High-resolution Mass Spectrometry
Flavonols
Liquid chromatography
Pharmaceutical Science
Counter-current chromatography
Hydrangea
Article
Secoriridoids
Analytical Chemistry
Drug Discovery
Stilbenes
ddc:66
ddc:6
Veröffentlichung der TU Braunschweig
Hydrangea Macrophylla Ssp. Serrata
Glycosides
Physical and Theoretical Chemistry
Countercurrent Distribution
Chromatography
High Pressure Liquid
Nuclear magnetic resonance spectroscopy
Cyanogenic Glycosides
Dihydro-isocoumarins
Dihydro-stilbenoids
Organic Chemistry
Isocoumarins
Chemistry (miscellaneous)
Molecular Medicine
Publikationsfonds der TU Braunschweig
Hydrangea macrophylla ssp. serrata
dihydro-isocoumarins
dihydro-stilbenoids
cyanogenic glycosides
flavonols
secoriridoids
counter-current chromatography
liquid chromatography
ion-mobility high-resolution mass spectrometry
nuclear magnetic resonance spectroscopy
Zdroj: Molecules : Special Issue Analytical and Preparative Hyphenation of Countercurrent Chromatography (CCC/CPC) to Structure Elucidation Methods in the Field of Natural Product Recovery and Identification 27 (2022) 11, 3424.-https:// doi.org/10.3390/molecules27113424--Molecules : a journal of synthetic chemistry and natural product chemistry / Molecular Diversity Preservation International--1420-3049--1420-3049
Molecules; Volume 27; Issue 11; Pages: 3424
DOI: 10.3390/molecules27113424
Popis: Previously, different Hydrangea macrophylla ssp. serrata cultivars were investigated by untargeted LC-MS analysis. From this, a list of tentatively identified and unknown compounds that differ significantly between these cultivars was obtained. Due to the lack of reference compounds, especially for dihydro-isocoumarins, we aimed to isolate and structurally characterise these compounds from the cultivar ‘Yae-no-amacha’ using NMR and LC-MS methods. For purification and isolation, counter-current chromatography was used in combination with reversed-phase preparative HPLC as an orthogonal and enhanced purification workflow. Thirteen dihydro-isocoumarins in combination with other metabolites could be isolated and structurally identified. Particularly interesting was the clarification of dihydrostilbenoid glycosides, which were described for the first time in H. macrophylla ssp. serrata. These results will help us in further studies on the biological interpretation of our data.
Databáze: OpenAIRE
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