Gene expression analysis during platelet-like particle production in phorbol myristate acetate-treated MEG-01 cells
| Autor: | Takao Taki, Takuma Kawakami, Yoshimasa Isakari, Hiroshi Kiwada, Shinji Sogo, Toshihide Ono, Tatsuhiro Ishida |
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| Rok vydání: | 2009 |
| Předmět: |
Pharmacology
Blood Platelets Microarray analysis techniques Reverse Transcriptase Polymerase Chain Reaction Megakaryocyte differentiation Gene Expression Profiling Cell Pharmaceutical Science General Medicine Biology Molecular biology Cell Line Gene expression profiling medicine.anatomical_structure Megakaryocyte Cell culture Tetradecanoylphorbol Acetate Gene expression medicine Humans Megakaryocytes Oligonucleotide Array Sequence Analysis |
| Zdroj: | Biologicalpharmaceutical bulletin. 32(3) |
| ISSN: | 0918-6158 |
| Popis: | A comprehensive gene-expression analysis during platelet (PLT) production from megakaryocytes may give important information on genes involved in the PLT production process. However, the low abundance of primary megakaryocytes makes the gene expression analysis difficult. Therefore, we employed MEG-01 cells, a human megakaryocytic cell line, and confirmed that the cell line produces PLT-like particles by treatment with phorbol myristate acetate (PMA). After treatment of MEG-01 cells with PMA for 8 or 24 h, comprehensive gene expression analysis was carried out using a microarray and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). From the microarray analysis, 141 genes were up-regulated (>2-fold) and 164 genes were down-regulated ( |
| Databáze: | OpenAIRE |
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