Bcl-2 inhibits the caspase-dependent apoptosis induced by SARS-CoV without affecting virus replication kinetics
| Autor: | Licia Bordi, Concetta Castilletti, Mauro Piacentini, Giuseppe Ippolito, Alessandra Rinaldi, Maria Rosaria Capobianchi, Fabiola Ciccosanti, Gabriella Rozera, S. Calcaterra, Laura Falasca, S. Zaniratti, A. Di Caro |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Programmed cell death
Time Factors viruses Infectious Disease Apoptosis Virus Replication Caspase-Dependent Apoptosis Virus Multiplicity of infection PARP Cleavage Virology Chlorocebus aethiops Animals Vero Cells Caspase biology Brief Report General Medicine Mitochondrial Pathway Viral replication Proto-Oncogene Proteins c-bcl-2 Severe acute respiratory syndrome-related coronavirus Caspases biology.protein Vero cell Vero Cell |
| Zdroj: | Archives of Virology |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Summary. Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway, as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells. |
| Databáze: | OpenAIRE |
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